Abstract
A filtration flow-through design was used to develop the rapid immunodetection of Escherichia coli. Polyclonal anti-E. coli IgG was conjugated to small, 0.8 μ Blue latex beads. Cells were mixed with conjugated beads in the presence of anti-E. coli monoclonal IgM. The suspension was then filtered through a 5 μ nitrocellulose membrane. The cell-containing complexes were effectively collected on the filter, forming a blue spot. The method produced reliable detection of E. coli at a concentration of 105 cells ml−1, which is a current benchmark figure for urinary tract infection (UTI) diagnosis.
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References
Blum RN, Wright RA (1992) Detection of pyuria and bacteriuria in symptomatic ambulatory women. J. Gen. Int. Med. 7: 140–144.
Eisenstadt J, Washington JA (1996) Diagnostic microbiology for bacteria and yeast causing urinary tract infections. In: Mobley HLT, Warren JW, eds. Urinary Tract Infections, Molecular Pathogenesis and Clinical Management. Washington, DC: American Society for Microbiology, pp. 29–66.
Goers J (1993) Immunochemical Techniques Laboratory Manual. San Diego: Academic Press.
Hengstler KA, Hammann R, Fahr AM (1997) Evaluation of BBL CHROMagar orientation medium for detection and presumptive identification of urinary tract pathogens [see comments]. J. Clin. Microbiol. 35: 2773–2777.
Johnson JL, Brooke CL, Fritschel SJ (1998a) Comparison of the BAX for screening/E. coli O157:H7 method with conventional methods for detection of extremely low levels of Escherichia coli O157:H7 in ground beef. Appl. Environ. Microbiol. 64: 4390–4395.
Johnson JR, Brown JJ, Carlino UB, Russo TA (1998b) Colonization with and acquisition of uropathogenic Escherichia coli as revealed by polymerase chain reaction-based detection. J. Infect. Dis. 177: 1120–1124.
Samra Z, Heifetz M, Talmor J, Bain E, Bahar J (1998) Evaluation of use of a new chromogenic agar in detection of urinary tract pathogens. J. Clin. Microbiol. 36: 990–994.
Vernozy-Rozand C, Mazuy C, Ray-Gueniot S, Boutrand-Loei S, Meyrand A, Richard Y (1998) Evaluation of the VIDAS methodology for detection of Escherichia coli O157 in food samples. J. Food. Prot. 61: 917–20.
Warren JW (1996) Clinical presentations and epidemiology of urinary tract infections. In: Mobley HLT, Warren JW, eds. Urinary Tract Infections, Molecular Pathogenesis and Clinical Management. Washington, DC: American Society for Microbiology, pp. 3–27.
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Arredondo, A.R., Dorval, B.L., Klibanov, A.M. et al. Rapid immunodetection of Escherichia coli. Biotechnology Letters 22, 547–550 (2000). https://doi.org/10.1023/A:1005616523897
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DOI: https://doi.org/10.1023/A:1005616523897