Abstract
The production of a homogeneous liquid culture of mushroom mycelium with a high density of viable inoculum points is a prerequisite for the adaptation of the liquid culture technology to the mushroom spawn production process. Homogenisation proved unsuitable as a technique to produce a morphology of this nature because of the shear sensitive nature of Agaricus bisporus. To overcome this limitation, a homogeneous culture was produced by exposing culture flasks to alternating periods of shear stress (300 rpm on a shaker table for 60 min day−1) and recovery (23 h day−1 under static conditions).
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Friel, M.T., McLoughlin, A.J. Production of a liquid inoculum/spawn of Agaricus bisporus. Biotechnology Letters 22, 351–354 (2000). https://doi.org/10.1023/A:1005616516646
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DOI: https://doi.org/10.1023/A:1005616516646