Abstract
The kinetics of konjac powder hydrolysis by β-mannanase from Bacillus licheniformis was studied for the production of manno-oligosaccharides, a bifidobacteria factor. It is assumed that, during the whole reaction, the substrate has an average degree of polymerization with which the apparent kinetic constants, K m and k +2, vary and the concentration of substrate remains unchanged. Based on the above assumptions, a set of simple expressions is proposed to correlate the initial concentration of substrate, enzyme concentration, degree of hydrolysis and reaction time. The results predicted are in good agreement with the experimental data.
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References
Gomes J, Steiner W (1998) Production of a high activity of an extremely thermostable β-mannanase by the thermophilic eubacterium Rhodothermus marinus, grown on locust bean gum. Biotechnol. Lett. 20: 729-733.
Hossain MZ, Abe J, Hizukuri S (1996) Multiple forms of β-mannanase from Bacillus sp. KK01. Enzyme Microbiol. Technol. 18: 95-98.
McCleary BV, Matheson NK (1983) Action patterns and substratebinding requirements of β-D-mannanase with mannosaccharides and mannan-type polysaccharides. Carbohydr. Res. 119: 191-219.
Nikolov ZL, Meagher MM, Reilly PJ (1989) Kinetics, equilibria, and modeling of the formation of oligosaccharids from D-glucose with Aspergillus niger glucoamylases I and II. Biotechnol. Bioeng. 34: 694-704.
Rademacher TW, Parekh RB, Dwek RA (1988) Glycobiology. Annu. Rev. Biochem. 57: 785-838.
Rastall RA, Rees NH, Wait R, Adlard MW, Bucke C (1992) α-Mannosidase-catalysed synthesis of novel manno-, lyxo-, and heteromanna-oligosaccharides: a comparison of kinetically and thermodynamically mediated approaches. Enzyme Microbiol. Technol. 14: 53-57.
Rättö M, Poutanen K (1988) Production of mannan-degrading enzymes. Biotechnol. Lett. 10: 661-664.
Suwasono S, Rastall RA (1998) Synthesis of oligosaccharides using immobilised 1,2-α-mannosidase from Aspergillus phoenicis: immobilisation-dependent modulation of product spectrum. Biotechnol. Lett. 20: 15-17.
Valette P, Pelene V, Djouzi Z, Paul F, Monsan P, Szylit O (1993) Bioavailability of new synthesised glucooligosaccharides in the intestinal tract of gnotobiotic rats. J. Sci. Food Agric. 62: 121-127.
Voragen AGJ (1998) Technological aspects of functional foodrelated carbohydrates. Trends Food Sci. Technol. 9: 328-335.
Zhang J, He ZM, Hu K (2000) Purification and characterization of β-mannanase from Bacillus licheniformis for industrial use. Biotechnol. Lett. 22: 1375-1378.
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He, Z., Zhang, J. & Huang, D. A kinetic correlation for konjac powder hydrolysis by β-mannanase from Bacillus licheniformis. Biotechnology Letters 23, 389–393 (2001). https://doi.org/10.1023/A:1005615204340
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DOI: https://doi.org/10.1023/A:1005615204340