Abstract
The accumulation of biofilm by Acetobacterium sp. during continuous culture in an upflow anaerobic filter (UAF) growing on methanol-formate was the result of space velocity and inlet concentrations of substrate and Co+2. To achieve good development of biofilm, a space velocity of 0.38 h−1, inlet substrate concentrations of 125 mM of both methanol and formate, and Co+2 at 0.16 mM were required. Cell productivities in the effluent of the UAF-reactor were about 6-fold higher than in chemostat cultures (0.20 g l−1 h−1 for UAF and 0.035 g l−1 h−1 for chemostat) (previous studies), and the maximum vitamin B12 specific concentration was 5.1 mg g cell−1.
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Bainotti, A.E., Estebanez, B., Nagadomi, H. et al. Production of vitamin B12 in an upflow anaerobic filter continuous reactor using Acetobacterium sp.. Biotechnology Letters 22, 503–508 (2000). https://doi.org/10.1023/A:1005612819257
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DOI: https://doi.org/10.1023/A:1005612819257