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Cloning of a dextransucrase gene (fmcmds) from a constitutive dextransucrase hyper-producing Leuconostoc mesenteroides B-512FMCM developed using VUV

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Abstract

Dextransucrase (FMCMDS) from Leuconostoc mesenteroides B-512FMCM, a dextransucrase constitutive and hyper-producing strain, catalyzes the synthesis of dextran from sucrose. The coding region for fmcmds was isolated and sequenced. It consisted of an open reading frame (ORF) of 4699 bp, coding for a 1527 amino acid protein with a molecular mass of 170 kDa. However, it showed a dextransucrase activity band at 180 kDa in SDS-PAGE. Only one nucleotide changed in the promoter site and two amino acid residues were changed in the structural gene from that of the parent L. mesenteroides NRRL B-512F dsrS; an inducible dextransucrase gene of low productivity.

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Authors and Affiliations

  1. Department of Fine Chemical Engineering, Chonnam National University, Kwangju, 500-757, Korea

    Hwa-Ja Ryu

  2. Department of Fine Chemical Engineering, Department of Biochemical Engineering and Research Institute for Catalysis, Chonnam National University, Kwangju, 500-757, Korea

    Doman Kim

  3. Research Center for New Biomaterial in Agriculture, Seoul National University, Suwon, 441-744, Korea

    Doman Kim

  4. Department of Physics, Kangnung National University, Kangnung, 210-702, Korea

    Do-Won Kim

  5. Department of Botany, Iowa State University, Ames, IA, 50011, USA

    You-Youn Moon

  6. Laboratory of Carbohydrate Chemistry and Enzymology, Iowa State University, Ames, IA, 50011, USA

    John F. Robyt

Authors
  1. Hwa-Ja Ryu
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  2. Doman Kim
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  3. Do-Won Kim
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  4. You-Youn Moon
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  5. John F. Robyt
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Ryu, HJ., Kim, D., Kim, DW. et al. Cloning of a dextransucrase gene (fmcmds) from a constitutive dextransucrase hyper-producing Leuconostoc mesenteroides B-512FMCM developed using VUV. Biotechnology Letters 22, 421–425 (2000). https://doi.org/10.1023/A:1005609718173

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