Abstract
A hairy root line from Catharanthus roseus was cultured in a 14 l bioreactor. Nitrate and phosphate uptakes were similar to the same root line cultured in a 250 ml Erlenmeyer flask. However, sucrose consumption rate was slower in roots cultured in the bioreactor. These results show that it is feasible to upgrade this hairy root line to bioreactor level and, although carbon utilization has to be improved, ajmalicine and catharanthine were still produced and were retained within the biomass tissue.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bhadra R, Shanks JV (1997) Transient studies of nutrient uptake, growth, and indole alkaloid accumulation in heterotrophic cultures of hairy roots of Catharanthus roseus. Biotechnol. Bioeng. 55: 527–534.
Ciau-Uitz R, Miranda-Ham ML, Coello-Coello J, Chí B, Pacheco LM, Loyola-Vargas VM (1994) Indole alkaloid production by transformed and non-transformed root cultures of Catharanthus roseus. In Vitro Cell Dev. Biol. 30P: 84–88.
Courtois D, Guern J (1980) Temperature response of Catharanthus roseus cells cultivated in liquid medium. Plant Sci. Lett. 17: 473–482.
Gomori G (1942) J. Lab. Clin. Med. 27: 955–960.
Jung KH, Kwak SS, Kim SW, Lee H, Choi CY, Liu JR (1992) Improvement of catharanthine productivity in hairy root cultures of C. roseus by using monosaccharides as carbon source. Biotechnol. Lett. 14: 695–700.
Kreis W, Reinhard E (1989) The production of secondary metabolites by plant cells cultivated in bioreactors. Planta Med. 55: 409–416.
Monforte-González M, Ayora-Talavera T, Maldonado-Mendoza IE, Loyola-Vargas VM (1992) Quantitative analysis of serpentine and ajmalicine in plant tissues of Catharanthus roseus and hyoscyamine and scopolamine in root tissues of Datura stramonium by densitometry in thin layer chromatography. Phytochem. Anal. 3: 117–121.
Nicholas DJ, Nasson A (1971) Determination of nitrate and nitrite. Meth. Enzymol. 23: 491–503.
Toivonen L, Ojala M, Kauppinen V (1990) Indole alkaloid production by hairy root cultures of Catharanthus roseus: growth kinetics and fermentation. Biotechnol. Lett. 12: 519–524.
Vázquez-Flota F, Moreno-Valenzuela OA, Miranda-Ham ML, Coello-Coello J, Loyola-Vargas VM (1994) Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures. Medium optimization and elicitation. Plant Cell Tiss. Org. Cult. 38: 273–279.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Moreno-Valenzuela, O., Coello-Coello, J., Loyola-Vargas, V. et al. Nutrient consumption and alkaloid accumulation in a hairy root line of Catharanthus roseus . Biotechnology Letters 21, 1017–1021 (1999). https://doi.org/10.1023/A:1005606818744
Issue Date:
DOI: https://doi.org/10.1023/A:1005606818744