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Transmission of Helicobacter pylori in an Animal Model

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Abstract

An experimental murine model was studied to evaluate the orogastrointestinal colonization of Helicobacter pylori and the animal-to-animal transmission. Balb/C mice were infected with H. pylori and housed with uninoculated mice in cages with and without a grate on the floor. Mice were killed after 7, 14, 30, and 45 days, and samples from the esophagus, stomach, small intestine, colon, and rectum were analyzed for H. pylori by PCR and immunohistochemistry and for histological changes. Bacterial colonization was assessed also by culture from stomach samples. H. pylori was cultured by stomach samples of infected mice at 7, 14, and 30 days. Using PCR and immunohistochemistry, H. pylori was detected in inoculated and uninoculated mice in all areas examined, with an high percentage of positive samples in the esophagus and stomach. Moreover transmission was detected, without differences, regardless of whether mice were housed with or without a grate on the floor, supporting an orooral animal transmission.

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Authors and Affiliations

  1. Departments of Biomedical Sciences, Medicine, and Odontostomatology, Faculty of Medicine, University of Chieti, Italy

    Luigina Cellini, Leonardo Marzio, Giuseppina Ferrero, Alessandro Del Vino, Emanuela Di Campli, Laurino Grossi, Sonia Toracchio & Luciano Artese

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  1. Luigina Cellini
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  2. Leonardo Marzio
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  3. Giuseppina Ferrero
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  4. Alessandro Del Vino
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  5. Emanuela Di Campli
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  6. Laurino Grossi
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  7. Sonia Toracchio
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  8. Luciano Artese
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Cellini, L., Marzio, L., Ferrero, G. et al. Transmission of Helicobacter pylori in an Animal Model. Dig Dis Sci 46, 62–68 (2001). https://doi.org/10.1023/A:1005605724271

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