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Stabilisation of immunoconjugates by trehalose

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Abstract

Stable immunoconjugates were prepared in the presence of 400 mM trehalose. Their residual activity after freeze-drying, rehydration and incubation for 9 h at 40 °C was 35%. Freeze-dried conjugates containing 400 mM trehalose incubated at 40 °C for 4 days retained 80% of their original activity.

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Authors and Affiliations

  1. Lab. de Microesferas e Lipossomos, C. de Biotecnologia, I. Butantan, Av, Vital, Brasil, 1500

    Maria Izabel Esteves, Wagner Quintilio, Rony A. Sato, Isaías Raw & Maria Helena Bueno da Costa

  2. I. de Química – USP, Av. Prof. Lineu Prestes 748, 05508-900, São Paulo, SP, Brasil

    Pedro Soares de Araujo

Authors
  1. Maria Izabel Esteves
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  2. Wagner Quintilio
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  3. Rony A. Sato
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  4. Isaías Raw
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  5. Pedro Soares de Araujo
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  6. Maria Helena Bueno da Costa
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Esteves, M.I., Quintilio, W., Sato, R.A. et al. Stabilisation of immunoconjugates by trehalose. Biotechnology Letters 22, 417–420 (2000). https://doi.org/10.1023/A:1005605616356

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  • DOI: https://doi.org/10.1023/A:1005605616356

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