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Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E. coli

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Abstract

To study the characteristics of recombinant thin aggregative fimbriae of salmonella and to develop a vaccine for salmonella infections, the AgfA subunit gene was amplified from Salmonella entiritidis using PCR. Maltose binding protein (MBP)-AgfA fusion protein was over-produced in E. coli and purified. Antibody against MBP-AgfA was prepared and its immunogenicity was studied.

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Authors and Affiliations

  1. Cell Cycle and Signal Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea

    Misun Won

  2. Departments of Food Science and Technology, Korea

    Soyoun Kim, Seunghwan Lee & Kyung Bin Song

  3. Veterinary Medicine, Chungnam National University, Taejon, 305-764, Korea

    Chuljung Kim, Hyunsu Kim & Moohyung Jun

Authors
  1. Misun Won
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  2. Soyoun Kim
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  3. Seunghwan Lee
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  4. Chuljung Kim
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  5. Hyunsu Kim
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  6. Moohyung Jun
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  7. Kyung Bin Song
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Won, M., Kim, S., Lee, S. et al. Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E. coli. Biotechnology Letters 22, 1165–1168 (2000). https://doi.org/10.1023/A:1005605610004

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  • DOI: https://doi.org/10.1023/A:1005605610004

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