Abstract
An alternative method for the analysis of poly-β-hydroxybutyrate (PHB) in microbial biomass based on FTIR spectroscopy was developed in which a semi-transparent, thin pellet of biomass was made with KBr and scanned by IR spectroscopy, from 400 cm−1to 2000 cm−1. The IR spectra of the target biomass showed a distinct absorbance band of the carboxylic ester group at 1724.03 cm−1. The technique can be used to screen large numbers of microorganisms for PHB production.
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Misra, A., Thakur, M., Srinivas, P. et al. Screening of poly-β-hydroxybutyrate-producing microorganisms using Fourier transform infrared spectroscopy. Biotechnology Letters 22, 1217–1219 (2000). https://doi.org/10.1023/A:1005602911977
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DOI: https://doi.org/10.1023/A:1005602911977