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Effect of Peroxynitrite on Motor Function of the Opossum Esophagus

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Abstract

Nitric oxide (NO·) is a mediator of esophageal motility. Esophageal dysmotility accompanies esophagitis. During inflammation, superoxide and NO· form peroxynitrite (ONOO-), a reactive molecule that alters cellular function. We tested the hypotheses that ONOO- affects esophageal motility and is produced in association with esophagitis. Transverse muscle strips from the opossum esophagus were stimulated by an electrical field, and nitrotyrosine immunoblots were performed. Peroxynitrite, its decomposed form, or NaNO2 relaxed the lower esophageal sphincter (LES) and attenuated the off response. These effects were inhibited by oxyhemoglobin (Hgb). An antagonist of guanylate cyclase, 1H-[1,2,4]oxadiazole[4,3]quinoxalin-1-one (ODQ), inhibited the LES relaxation produced by ONOO-. Nitrotyrosine, a footprint for ONOO- production, was detected in inflamed esophagus. These studies support the hypotheses that ONOO- alters esophageal motor function and is formed in association with esophagitis. It is possible that some of the esophageal motor dysfunction seen with esophagitis may be related to the formation of ONOO-.

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Authors and Affiliations

  1. Departments of Internal Medicine and Pediatrics, University of Iowa College of Medicine, USA and

    Aliye Uc, Joseph A. Murray, Neil Kooy & Jeffrey L. Conklin

  2. Department of Veterans Affairs Medical Center, Iowa City, Iowa, 52242

    Aliye Uc

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  1. Aliye Uc
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  2. Joseph A. Murray
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  3. Neil Kooy
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  4. Jeffrey L. Conklin
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Uc, A., Murray, J.A., Kooy, N. et al. Effect of Peroxynitrite on Motor Function of the Opossum Esophagus. Dig Dis Sci 46, 30–37 (2001). https://doi.org/10.1023/A:1005596328395

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