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Mutagenic Analysis of the F 0 Stator Subunits

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Abstract

The a and b subunits constitute the stator elements in the F0 sector of F1F0-ATP synthase.Both subunits have been difficult to study by physical means, so most of the information onstructure and function relationships in the a and b subunits has been obtained using mutagenesisin combination with biochemical methods. These approaches were used to demonstrate thatthe a subunit in association with the ring of c subunits houses the proton channel throughF1F0-ATP synthase. The map of the amino acids contributing to the proton channel is probablycomplete. The two b subunits dimerize, forming an extended flexible unit in the peripheralstalk linking the F1 and F0 sectors. The unique characteristics of specific amino acid substitutionsaffecting the a and b subunits suggested differential effects on rotation during F1F0-ATPaseactivity.

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  1. Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, Gainesville, Florida, 32610

    Brian D. Cain

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Cain, B.D. Mutagenic Analysis of the F 0 Stator Subunits . J Bioenerg Biomembr 32, 365–371 (2000). https://doi.org/10.1023/A:1005575919638

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