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Kinetics of cell growth and secondary metabolism of a high-tanshinone-producing line of the Ti transformed Salvia miltiorrhiza cells in suspension culture

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Abstract

When cultivated in 6,7-V medium in suspension culture, Salvia miltiorrhiza, transformed with Agrobacterium tumefaciens C58, grew rapidly, reaching about 9.7 g l−1 dry wt after 12 days. The cell line produced tanshinones: 150 mg cryptotanshinone, 20 mg tanshinone I and 50 mg tanshinone IIA/l and phenolic acids: 530 mg rosmarinic acid and 216 mg lithospermic acid B/l. The phenolic acids were intracellular while about 1/3 of the tanshinones were extracellular. This is the first report of simultaneous production of both phenolic acids and tanshinones in a single culture system.

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Authors and Affiliations

  1. Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong

    Hui Chen & Feng Chen

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  1. Hui Chen
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  2. Feng Chen
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Chen, H., Chen, F. Kinetics of cell growth and secondary metabolism of a high-tanshinone-producing line of the Ti transformed Salvia miltiorrhiza cells in suspension culture. Biotechnology Letters 21, 701–705 (1999). https://doi.org/10.1023/A:1005562410037

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  • DOI: https://doi.org/10.1023/A:1005562410037

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