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Synthesis of α-galacto-oligosaccharides by a cloned α-galactosidase from Bifidobacterium adolescentis

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Abstract

A genomic library of Bifidobacterium adolescentis was constructed in Escherichia coli and a gene encoding an α-galactosidase was isolated. The identified open reading frame showed high similarity and identity with bacterial α-galactosidases, which belong to Family 36 of the glycosyl hydrolases. For the purification of the enzyme from the medium a single chromatography step was sufficient. The yield of the recombinant enzyme was 100 times higher than from B. adolescentis itself. In addition to hydrolytic activity the α-galactosidase showed transglycosylation activity and can be used for the production of α-galacto-oligosaccharides.

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Authors and Affiliations

  1. Food Chemistry chair, Division of Food Science, Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, PO Box 8129, 6700, EV Wageningen, The Netherlands

    L.A.M. van den Broek, J. Ton, J.C. Verdoes, K.M.J. Van Laere, A.G.J. Voragen & G. Beldman

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  1. L.A.M. van den Broek
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  2. J. Ton
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  3. J.C. Verdoes
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  4. K.M.J. Van Laere
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  5. A.G.J. Voragen
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  6. G. Beldman
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van den Broek, L., Ton, J., Verdoes, J. et al. Synthesis of α-galacto-oligosaccharides by a cloned α-galactosidase from Bifidobacterium adolescentis . Biotechnology Letters 21, 441–445 (1999). https://doi.org/10.1023/A:1005542521708

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  • DOI: https://doi.org/10.1023/A:1005542521708

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