Abstract
The influence of ampicillin and chloramphenicol administered intraperitoneallysingly or in combination on the protein content and the activities of hepaticsterase and amidase have been investigated in rats. The results have beencompared to the effects of phenobarbitone (inducer) andp-nitrophenyl-phosphate (inhibitor) of hepatic hydrolases.
Ampicillin pretreatment reduced protein level and amidase activity by3.5% each but caused a significant increase (8.1%) in total esteraseactivity compared to controls. Chloramphenicol treatment caused an overalldecrease in protein level, esterase and amidase activities respectively by11%, 11%, and 35% over controls.
Combined administration of both drugs resulted in a decrease in protein,esterase and amidase activities by 11.5%, 12.5%, and 41.2% respectively,thus mimicking the effects obtained with chloramphenicol alone.
The changes induced by administration of the drugs particularly incombination on the constituent enzymes of rat hepatic hydrolases may affectthe ability of the body to deal with exposure to environmental chemicals ifextrapolated to man.
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REFERENCES
Krisch, K. (1971) Carboxy ester hydrolases. In: The Enzymes (P. D. Boyer, edn.), 3rd ed., Vol. 5. Academic Press, New York, pp. 43–69.
Mentlein, R., Heiland, S., and Heymann, E. (1980) Arch. Biochem. Biophys. 200:547–559.
Kaur, S., and Ali, B. (1982) Res. Comm. Chem. Pathol. Pharmacol. 38:343–344.
Satoh, T., and Moroi, K. (1973) Life Sci. 12:169–176.
Silver, E. H., and Murphy, S. D. (1981) Toxicol. Appl. Pharmacol. 57:208–219.
Ali, B., and Kaur, S. (1982) Biochem. Pharmacol. 31:3683–3684.
Annual Report of the Working Party on Pesticide Residues: 1994 (1995) MAFF & HSE. Supplement to the Pesticide Register. HMSO, London, pp. vii and 154.
Food and Chemical Toxicology (1996) Information Section: Pesticide Residues in Food 34:317–325.
McCracken, N. W., Blain, P. G., and Williams, F. M. (1993) Biochem. Pharmacol. 45:31–36.
Nwankwo, J. O., Adebayo, J. A., and Emerole, G. O. (1996) Afr. J. Med. Med. Sci. 25:111–115.
Holt, D. E., Ryder, T. A., Fairbairn, A., Hurley, R., and Harvey, D. (1997) Human Exp. Toxicol. 116:570–576.
Holt, D. E., Andrews, C. M., Pyne, J. P., Williams, T. C., and Turton, J. A. (1998) Human Exp. Toxicol. 17:8–17.
Kaplan, S. L., and Feigin, R. D. (1983) Ped. Clin. North. Amer. 30:259–269.
Bradley, J. S., and Scheld, W. M. (1997) Clin. Infec. Disease 24:213–221.
Akpede, O., Abiodun, P. O., Sykes, M., and Salami, C. E. (1994) East Afr. Med. J. 71:14–20.
Remmer, H., and Merker, H. J. (1963) Science 142:1657–1658.
Trinder, P. (1954) Biochem. J. 57:301–303.
Kaur, S., and Ali, B. (1983) Toxicol. Appl. Pharmacol. 70:156–159.
Lowry, O. H., Rosenbrough, N. J., Farr, L., and Randall, R. J. (1951) J. Biol. Chem. 193:265–275.
Rasoanaivo, P. et al. (1994) Planta Med. 60:13–16.
Sowunmi, A. M., Oduola, A. M. J., Ogundahunsi, O. A. T., Falade, C. O., Gbotosho, G. O., and Salako, L. A. (1997) Trans. Roy. Soc. Trop. Med. Hyg. 91:63–67.
Nwankwo, J. O., Garba, M. A., Chinje, C. E., Mgbojikwe, L. O., and Emerole, G. O. (1990) Biochem. Pharmacol. 40:654–659.
Brandt, E., Hayman, E., and Mentlein, R. (1980) Biochem. Pharmacol. 29:1927–1931.
Pohl, L. R., and Krishna, G. (1977) Biochem. Pharmacol. 27:335–341.
Siddiqui, M. K. J., and Walker, C. H. (1996) Pest. Sci. 48:141–148.
Conney, A. H., Davison, C., Gastel, R., and Burns, J. J. (1960) J. Pharmacol. Exp. Ther. 130:1–8.
Howes, J. F., and Hunter, W. H. (1968) J. Pharm. Pharmacol. 20:107–110.
Moroi, K., and Satoh, T. (1975) Biochem. Pharmacol. 24:1436–1437.
Conney, A. H., and Gilman, A. G. (1963) J. Biol. Chem. 238:3682–3689.
Garrod, L. P., Lambert, H. P., O'Grady, F., and Waterworth, P. M. (1981) In: Antibiotics and Chemotherapy, 5th edn., Churchill Livingstone, New York.
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Farombi, E.O., Nwankwo, J.O., Wara, S.H. et al. Chloramphenicol and Ampicillin-Induced Changes in Rat Hepatic Esterase and Amidase Activities. Biosci Rep 20, 13–19 (2000). https://doi.org/10.1023/A:1005527032317
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DOI: https://doi.org/10.1023/A:1005527032317