Skip to main content
SpringerLink
Log in

Economic improvement of the fermentative production of gallidermin by Staphylococcus gallinarum

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Gallidermin production by Staphylococcus gallinarum was optimal using a new developed medium consisting of 50 g yeast extract l−1, 45 g CaCl2 l−1 and 5 g maltose l−1. Medium optimisation led to the cost of the medium decreasing by 93% compared to the previously used medium and in addition gained a higher product yield. A scale-up of the process based on the new medium was successfully tested up to a working volume of 200 l.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bierbaum G, Götz F, Peschel A, Kupke T, van de Kamp M, Sahl H-G (1996) The biosynthesis of the lantibiotics epidermin, gallidermin, Pep5 and epilancin K7. Antonie von Leeuwenhoek 69: 119-127.

    Google Scholar 

  • Dennesen PJW, Bonten MJM, Weinstein RA (1998) Multiresistant bacteria as a hospital epidemic problem. Ann. Med. 30: 176-185.

    Google Scholar 

  • Fiedler H-P, Hörner T, Wörn A (1987) Separation of polypeptide antibiotics by reversed-phase high-performance liquid chromatography. Chromatographia 24: 433-438.

    Google Scholar 

  • Goldberg DE (1989) Genetic Algorithms in Search, Optimization and Machine Learning. Reading, MA: Addison-Welsley Publishing.

    Google Scholar 

  • Hörner T, Ungermann V, Zähner H, Fiedler H-P, Utz R, Kellner R, Jung G (1990) Comparative studies on the fermentative production of lantibiotics by staphylococci. Appl. Microbiol. Biotechnol. 32: 511-517.

    Google Scholar 

  • Holland JH (1975) Adaption in Natural and Artificial Systems. Ann Arbor, MI: The University of Michigan Press.

    Google Scholar 

  • Jack RW, Bierbaum G, Sahl H-G (1998) Lantibiotics and Related Peptides. Berlin: Springer-Verlag.

    Google Scholar 

  • Kellner R, Jung G, Hörner T, Zähner H, Schnell N, Entian KD, Götz F (1988) Gallidermin: a new lanthionine-containing polypeptide antibiotic. Eur. J. Biochem. 177: 53-59.

    Google Scholar 

  • Kempf M, Theobald U, Fiedler H-P (1997) Influence of dissolved O2 on the fermentative production of gallidermin by Staphylococcus gallinarum. Biotechnol. Lett. 19: 1063-1065.

    Google Scholar 

  • Peschel A, Schnell N, Hille M, Entian K-D, Götz F (1997) Secretion of the lantibiotics epidermin and gallidermin: sequence analysis of the genes gdmT and gdmH, their influence on epidermin production and their regulation by EpiQ. Mol. Gen. Genetics 254: 312-318.

    Google Scholar 

  • Sahl H-G, Jack R, Bierbaum G (1995) Biosynthesis and biological activities of lantibiotics with unique post-translational modifications. Eur. J. Biochem. 230: 827-853.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Mikrobiologisches Institut, Universität Tübingen, Auf der Morgenstelle 28, D-72076, Tübingen, Germany

    Markus Kempf, Uwe Theobald & Hans-Peter Fiedler

Authors
  1. Markus Kempf
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Uwe Theobald
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hans-Peter Fiedler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kempf, M., Theobald, U. & Fiedler, HP. Economic improvement of the fermentative production of gallidermin by Staphylococcus gallinarum. Biotechnology Letters 21, 663–667 (1999). https://doi.org/10.1023/A:1005514716686

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005514716686

Search

Navigation