Abstract
Trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of headspace atmospheric oxygen is shown to produce the trimethylsilyl derivatives of pyruvic and 2-oxobutyric acids, along with 2-hydroxy-2-methylmalonic and 2-hydroxy-2-ethylmalonic acids, respectively. This may lead to overestimation of these keto acids, if they were not oximated in the original sample, and the mistaken reporting of the 2-hydroxymalonates.
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References
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Mamer, O.A., Choiniere, L., Boismenu, D. et al. Artefactual pyruvate and 2-oxobutyrate produced by trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of oxygen. J Inherit Metab Dis 22, 821–826 (1999). https://doi.org/10.1023/A:1005510224963
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DOI: https://doi.org/10.1023/A:1005510224963