Abstract
An obligate alkalophilic Bacillus sphaericus strain, isolated from alkaline soils in the Himalaya, produced an extracellular protease which was optimally active at 50–55 °C and pH 10.5. The enzyme was stable in presence of 500 mg chlorine l−1 and as a detergent additive. Its stability in presence of laundry detergents was comparable to that of commercial proteases. The gelatin layer in 25 g of used X-ray films was efficiently hydrolyzed within 12 min at 50 °C, pH 11.0 and 25 U protease/ml.
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Singh, J., Vohra, R. & Sahoo, D. Alkaline protease from a new obligate alkalophilic isolate of Bacillus sphaericus . Biotechnology Letters 21, 921–924 (1999). https://doi.org/10.1023/A:1005502824637
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DOI: https://doi.org/10.1023/A:1005502824637