Skip to main content
Log in

Characterisation of osmotolerant hybrids obtained by fusion between protoplasts of Saccharomyces cerevisiae and heat treated protoplasts of Torulaspora delbrueckii

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Saccharomyces cerevisiae was fused with heat-treated protoplasts of an osmotolerant yeast, Torulaspora delbrueckii, to obtain hybrids having increased tolerance to increased glucose concentrations (up to 700 g l−1). The production of glycerol and arabitol by the hybrids was within the range of those of the parental strains, but the production of ethanol was higher.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adler L, Blomberg A, Nilsson A (1985) J. Bacteriol. 162: 300–306.

    Google Scholar 

  • Björkqvist S, Ansell R, Adler L, Liden G (1997) Appl. Environ. Microbiol. 63: 128–132.

    Google Scholar 

  • Deak T, Beuchat LR (1993) J. Food Protection 56: 777–782.

    Google Scholar 

  • Heluane H, Spencer JFT, Spencer D, Figueroa L de, Callieri DA (1993) Appl. Microbiol. Biotechnol. 40: 98–100.

    Google Scholar 

  • Hockney RC, Freeman RF (1980) Construction of polysaccharide-degrading brewing yeast by protoplast fusion. In: Ferenczy L, Farkas GL, eds. Advances in Protoplasts Research. Oxford: Pergamon Press, pp. 139–144.

    Google Scholar 

  • Infantes M, Schmidt JL (1992) Sci. Aliments 12: 271–287.

    Google Scholar 

  • Legmann R, Margalith P (1983) Eur. J. Appl. Microbiol. Biotechnol. 18: 320–322.

    Google Scholar 

  • Loray MA, Spencer JFT, Spencer DM, Figueroa LIC de (1995) J. Ind. Microbiol. 14: 508–513.

    Google Scholar 

  • Myers DK, Lambor DTM, Attfield PV (1997) Appl. Envion. Microbiol. 63: 145–150.

    Google Scholar 

  • Oda Y, Tonomura K (1995) Lett. Appl. Microbiol. 21: 190–193.

    Google Scholar 

  • Ohshima Y, Sugaura T, Horita M, Sasaki T (1987) Appl. Environ. Microbiol. 53: 1512–1514.

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual (2nd ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 6.50–6.58.

    Google Scholar 

  • Sanni AI, Loenner C (1993) Food Microbiol. 10: 517–523.

    Google Scholar 

  • Sasaki T, Ohshima Y (1987) Appl. Environ. Microbiol. 53: 1504–1511.

    Google Scholar 

  • Spencer JFT, Spencer DM (1978) Production of polyhydroxy alcohols by osmotolerant yeasts. In: Rose AH, ed. Primary Products of Metabolism. New York: Academic Press, pp. 393–425.

    Google Scholar 

  • Spencer JFT, Spencer DM (1997) Taxonomy: the names of the yeasts. In: Spencer JFT, Spencer DM, eds. Yeasts in Natural and Artificial Habitats. Heidelberg: Springer-Verlag, pp. 11–32.

    Google Scholar 

  • Yagi T (1991) Microbios. 68: 109–117.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lucca, M., Loray, M., de Figueroa, L. et al. Characterisation of osmotolerant hybrids obtained by fusion between protoplasts of Saccharomyces cerevisiae and heat treated protoplasts of Torulaspora delbrueckii . Biotechnology Letters 21, 343–348 (1999). https://doi.org/10.1023/A:1005488808895

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005488808895

Navigation