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Identification and heterologous expression of the polyhydroxyalkanoate biosynthesis genes from Alcaligenes sp. SH-69

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Abstract

Polyhydroxyalkanoate (PHA) biosynthesis genes were cloned and characterized from Alcaligenes sp. SH-69 which can synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from a single carbon source. The DNA sequence analysis revealed two consecutive genes coding for PHA synthase and β-ketothiolase and the gene coding for acetoacetyl-CoA reductase located about 2-kbp downstream of the two genes. Recombinant Escherichia coli strains with the cloned PHA biosynthesis genes synthesized poly(3-hydroxybutyrate) in Luria-Bertani medium containing 2% glucose and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in M9 minimal medium supplemented with 1% glucose, 1 mM valine, and 2 mM threonine, which demonstrates that the PHA biosynthesis genes of Alcaligenes sp. SH-69 are functional in E. coli. © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Department of Microbiology, Chungnam National University, Taejon, Korea

    Il Lee, Ho Myoung Ryu, Moo Woong Kim, Young Ha Rhee & Jeong-Yoon Kim

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  1. Il Lee
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  2. Ho Myoung Ryu
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  3. Moo Woong Kim
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  4. Young Ha Rhee
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  5. Jeong-Yoon Kim
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Lee, I., Ryu, H.M., Kim, M.W. et al. Identification and heterologous expression of the polyhydroxyalkanoate biosynthesis genes from Alcaligenes sp. SH-69. Biotechnology Letters 20, 969–975 (1998). https://doi.org/10.1023/A:1005458210991

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  • DOI: https://doi.org/10.1023/A:1005458210991

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