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Increased thermal stability of glucose dehydrogenase by cross-linking chemical modification

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Abstract

A hetero-oligomeric glucose dehydrogenase (GDH) from a moderate thermophilic bacterium, SM4 was cross-linked with glutaraldehyde (GA) and it now showed only one optimum temperature for reaction at around 65 °C, which approximately follows the Arrhenius equation. The native enzyme had shown optima at both 45 °C and 75 °C. In addition to the alteration of the optimum temperature for reaction, GA cross-linked GDH retained more than 90% of its initial activity even after 30 min of incubation at 65 °C.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-machi, Koganei, Tokyo, 184-8588, Japan

    Tomohiko Yamazaki, Wakako Tsugawa & Koji Sode

Authors
  1. Tomohiko Yamazaki
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  2. Wakako Tsugawa
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  3. Koji Sode
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Yamazaki, T., Tsugawa, W. & Sode, K. Increased thermal stability of glucose dehydrogenase by cross-linking chemical modification. Biotechnology Letters 21, 199–202 (1999). https://doi.org/10.1023/A:1005455911753

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  • DOI: https://doi.org/10.1023/A:1005455911753

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