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Synthesis of 2-keto-L-gulonic acid from gluconic acid by co-immobilized Gluconobacter oxydans and Corynebacterium sp.

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Abstract

2-Keto-L-gulonic acid was produced from gluconic acid using co-immobilized cells of Gluconobacter oxydans and Corynebacterium sp. with 2,5-diketo-D-gluconic acid. Gluconobacter oxydans and Corynebacterium sp. were entrapped together with polyvinylalcohol and alginate. 50 g/l glucose, 50 g/l gluconic acid, and the mixture of equal volume of 50 g/l glucose and 50 g/l gluconic acid were used as substrates. When the ratio of two cells was 1 to 1 with 100 mg cells/ml, the conversion of 2-KLG from gluconic acid was 38% (g/g). © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Department of Pharmacy, Shandong Medical University, Jinan Shandong, China

    Ji Aiguo

  2. State Key Laboratory of Microbial Technology, Shandong University, Jinan Shandong, China

    Gao Peiji

Authors
  1. Ji Aiguo
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  2. Gao Peiji
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Aiguo, J., Peiji, G. Synthesis of 2-keto-L-gulonic acid from gluconic acid by co-immobilized Gluconobacter oxydans and Corynebacterium sp.. Biotechnology Letters 20, 939–942 (1998). https://doi.org/10.1023/A:1005449909173

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  • DOI: https://doi.org/10.1023/A:1005449909173

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