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Growth kinetics and astaxanthin production of Phaffia rhodozyma on glycerol as a carbon source during batch fermentation

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Abstract

Glycerol was studied as a substrate for astaxanthin by Phaffia rhodozyma PR 190. With co-utilisation of yeast extract and peptone, the maximum specific growth rate was 0.24 ± 0.02 h−1. Astaxanthin percentage in total pigment is constant (0.78 mg/g) and its yield from glycerol is always 0.97 mg/g. The yield of biomass from glycerol alone is 0.50 ± 0.02 g/g. The specific rate of astaxanthin production versus the cell growth rate reached a maximum for an optimal specific growth rate of 0.075 h−1. For this optimal value, the maximum specific astaxanthin production rate is 0.09 ± 0.01 mg/g.h. The best astaxanthin results were : 33.7 mg/l, 0.2 mg/l.h and 1.8 mg/g yeast after a fermentation term of 168 hours. Our results suggest a strategy of astaxanthin production in fed batch culture or chemostat at a growth rate of 0.075 h−1. © Rapid Science Ltd. 1998

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References

  • Andrewes, A.G., Phaff, H.J., and Starr, M.P. (1976). Phytochemistry 15, 1003–1007

    Google Scholar 

  • An, G-H, Bielich, J., Auerbach, R., and Johnson, E.A. (1991). Bio/Technol. 9, 70–73

    Google Scholar 

  • An, G-H, Schuman, D.B., and Johnson, E.A. (1989). Appl. Environ. Microbiol. 55, 116–124

    Google Scholar 

  • Calo, P., De Miguel, T., Velaquez, J.B., and Villa, T.G. (1995). Biotechnol. Lett. 17, 575–578

    Google Scholar 

  • Haard, N.F. (1988). Biotechnol. Lett. 10, 609–614

    Google Scholar 

  • Hayman, G.T., Mannarelli, B.M., and Leathers, T.D. (1995). J. Indust. Microb. 14, 389–395

    Google Scholar 

  • Johnson, E.A., Villa, T.G., and Lewis, M.J. (1980). Aquaculture 20, 123–134

    Google Scholar 

  • Johnson, E.A., and Lewis, M.J. (1979). J. Gen. Microbiol. 115, 173–183

    Google Scholar 

  • Meyer, P.S., Du Preez, J.C., and Kilian, S.G. (1993). World J. Microbiol. Biotechnol. 9, 514–520

    Google Scholar 

  • Meyer, P.S., Du Preez, J.C. (1994). Appl. Microbiol. Biotechnol. 40, 780–785

    Google Scholar 

  • Sedmak, J.J., Weerasinghe, D.K., and Jolly, S.O. (1990). Biotechnol. Tech. 4, 107–112

    Google Scholar 

Download references

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Authors and Affiliations

  1. Département de Génie Biochimique et Alimentaire, Centre de Bioingénierie Gilbert Durand, UMR-CNRS, L.A. INRA, Institut National des Sciences Appliquées, Complexe Scientifique de Rangueil, Toulouse, France

    Endang Kusdiyantini, Philippe Gaudin, Gérard Goma & Philippe J. Blanc

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  1. Endang Kusdiyantini
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  2. Philippe Gaudin
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  3. Gérard Goma
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  4. Philippe J. Blanc
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Kusdiyantini, E., Gaudin, P., Goma, G. et al. Growth kinetics and astaxanthin production of Phaffia rhodozyma on glycerol as a carbon source during batch fermentation. Biotechnology Letters 20, 929–934 (1998). https://doi.org/10.1023/A:1005445808265

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  • DOI: https://doi.org/10.1023/A:1005445808265

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