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Increased Respiration Through Cytochrome d Enhances Microaerobic N2Fixation in Klebsiella Pneumoniae

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Abstract

Nitrogenase activity was increased in a Klebsiella pneumoniae strain (FN27) producing higher amounts of cytochrome d than the wild-type strain. The increased production of cytochrome d in FN27 showed a positive effect on nitrogenase activity in cells cultured with glucose as carbon source at 1 kPa oxygen but a negative effect at higher O2concentrations. In cells cultured with pyruvate as carbon source, FN27 expressed higher activity of nitrogenase at all oxygen tensions tested when compared to the wild-type strain. This analysis shows that the over production of cytochrome d terminal oxidase improves nitrogen fixation in certain culture conditions.

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Authors
  1. Luis Francisco Comaduran
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  2. Francisco Lara
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  3. Mario Soberón
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Comaduran, L.F., Lara, F. & Soberón, M. Increased Respiration Through Cytochrome d Enhances Microaerobic N2Fixation in Klebsiella Pneumoniae. Biotechnology Letters 20, 489–493 (1998). https://doi.org/10.1023/A:1005444330329

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  • DOI: https://doi.org/10.1023/A:1005444330329

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