Abstract
A strain of Variovorax paradoxus degrading 2,4-dichlorophenoxyacetic acid (2,4-D) was isolated from the Dijon area (France) using continuous chemostat culture. This strain, designated TV1, grew on up to 5 mM 2,4-D and efficiently degraded the herbicide as sole carbon source as well as in presence of soil extracts. It also degraded phenol and 2-methyl, 4-chlorophenoxyacetic acid at 3 mM and 2,4-dichlorophenol at 1 mM. This organism contained a stable 200 kb plasmid, designated pTV1, which showed no similarity in its restriction pattern with the archetypal 2,4-D catabolic plasmid pJP4. However, pTV1 contained an 11 kb BamHI fragment which hybridized at low stringency with the 2,4-D degradative genes tfdA, tfdB and tfdR from pJP4. PTV1 partial tfdA sequence showed 77 % similarity with the archetypal tfdA gene sequence from Ralstonia eutropha JMP134. Tn5 mutagenesis confirmed the involvement of this gene in the 2,4-D catabolic pathway. © Rapid Science Ltd. 1998
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Appleyard, RK (1954). Genetics 39: 440
Don, RH and Pemberton, JM (1981). J. Bacteriol. 145: 681–686
Figurski, DH and Helinski, DR (1979). Proc. Natl. Acad. Sci. USA 76: 1648–1652
Fukumori, F and Hausinger, RP (1993). J. Bacteriol. 175: 2083–2086
Fulthorpe, R, McGowan, C, Maltseva, O, Holben, W and Tiedje, JM (1995). Appl. Environ. Microbiol. 61:3274–3281
Galimand, M, Vieille, C, Perroud, B, Onyeocha, I and Elmerich, C (1988). Advances in genetics of Azospirillum brasilense Sp7: use of Tn5 mutagenesis for gene mapping and identification. In: Azospirillum IV: Genetics, physiology, ecology, W. Kling-müller ed pp 1–7, Berlin: Springer Verlag
Girard, H and Rougieux, R (1967). Techniques de microbiologie agricole. Paris: Dunod
Higgins, DG and Sharp, PM (1988). Gene 73: 237–244
Holben, WE, Schroeter, BM, Calabrese, VGM, Olsen, RH, Kukor, JK, Biederbeck, VO, Smith, AE, and Tiedje, JM (1992). Appl. Microbiol. Environ. 58:3941–3948.
Ka, JO and Tiedje, JM (1994). J. Bacteriol. 176: 5284–5289.
Loos, MA (1976). Can. J. Microbiol. 21: 104–107.
Mae, AA, Martis, RO, Ausmees, NR, Koiv, VM and Heinaru, AL (1993). J. Gen. Microbiol. 139: 3165–3170.
Maltseva, OV, McGowan, C, Fulthorpe, R and Oriel, P (1996). Microbiology. 142: 1115–1122.
Mc Clure, NC, Weightman, AJ and Fry, JC (1989). Appl. Environ. Microbiol. 55: 2627–2634.
Perkins, EJ, Gordon, MP, Caceres, O and Lurquin, PF (1990). J. Bacteriol. 172: 2351–2359.
Pieper, DH, Reineke, W, Engesser, K and Knackmuss, HJ (1989). Arch. Microbiol. 151:365–371.
Sambrook, J, Fritsch, EF and Maniatis, T (1989). Molecular cloning, a laboratory manual, 2nd edn, New-York: Cold Spring Harbour Laboratory Press
Selvaraj. G and Iyer, VN (1984). J. Bacteriol. 158:580–589.
Streber, W, Timmis, KN and Zenk, MH (1987). J. Bacteriol. 169: 2950–2955.
Suwa, Y, Wright, A, Fukimori, F, Nummy, KA, Hausinger, RP, Holben, WE and Forney, LJ (1996). Appl. Environ. Microbiol. 62: 2464–2469.
Top, EM, Maltseva, OM and Forney, LJ (1996). Appl. Environ. Microbiol. 62:1470–1476
Vallaeys, T, Fulthorpe, RR, Wright, A and Soulas, G (1996). FEMS. Microbiol. Ecol. 20:163–172.
Vallaeys, T, Persello-Cartieaux, F, Rouard, N, Lors, C, Laguerre, G and Soulas, G (1997). FEMS Microb. Ecol. 24:269–278.
Wheatcroft, R and Williams, PA (1981). J. Gen. Microbiol. 124: 433–437.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vallaeys, T., Albino, L., Soulas, G. et al. Isolation and characterization of a stable 2,4-dichlorophenoxyacetic acid degrading bacterium, Variovorax paradoxus, using chemostat culture. Biotechnology Letters 20, 1073–1076 (1998). https://doi.org/10.1023/A:1005438930870
Issue Date:
DOI: https://doi.org/10.1023/A:1005438930870