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Cloning and nucleotide sequence of one of the most highly expressed genes, a pdcA homolog of Aspergillus nidulans, in Aspergillus oryzae

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Abstract

A homolog of Aspergillus nidulans pdcA that is probably one of the most highly expressed in Aspergillus oryzae ATCC 22788 was isolated, as measured by the frequency among randomly selected 324 expressed sequence tags. It has an 1,632 bp open reading frame for a polypeptide of about 60 kDa. Its amino acid sequence revealed 74% identity and 84% similarity to that of A. nidulans pyruvate decarboxylase.

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Authors and Affiliations

  1. Institute for Molecular Biology and Genetics, Chonbuk National University, Chonju, 561–756

    Dong Whan Lee, Jeong-Shin Koh & Keon-Sang Chae

  2. Institute of Life Science, and Department of Food Science and Engineering, Woosuk University, Chonju, 565–701, Republic of Korea

    Jong Hwa Kim

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  1. Dong Whan Lee
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  2. Jeong-Shin Koh
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  3. Jong Hwa Kim
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  4. Keon-Sang Chae
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Lee, D.W., Koh, JS., Kim, J.H. et al. Cloning and nucleotide sequence of one of the most highly expressed genes, a pdcA homolog of Aspergillus nidulans, in Aspergillus oryzae . Biotechnology Letters 21, 139–142 (1999). https://doi.org/10.1023/A:1005438505623

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  • DOI: https://doi.org/10.1023/A:1005438505623

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