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Cloning and sequencing of a Deleya marina gene encoding for alginate lyase

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Abstract

A bacterial strain N-1 was isolated as a decomposer of alginate and identified as Deleya marina. The alyA encoding for alginate lyase was cloned into Escherichia coli. The structural gene, located on a 1.9-kb SalI fragment, revealed 1,122 bp encoding a mature protein of 348 amino acids and a signal peptide of 26 amino acids. The deduced amino acid sequence of the D. marina alginate lyase showed high homology to AlgL of Pseudomonas aeruginosa with 63% identity and belonging to class 1 by hydrophobic cluster analysis.

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Authors and Affiliations

  1. Department of Molecular chemistry, Graduate School of Engineering, Hokkaido University, N-13, W-8, Kita-ku, Sapporo, Hokkaido , 060-8628, Japan

    J. Kraiwattanapong, H. Tsuruga, T. Ooi & S. Kinoshita

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  1. J. Kraiwattanapong
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  2. H. Tsuruga
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  3. T. Ooi
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  4. S. Kinoshita
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Kraiwattanapong, J., Tsuruga, H., Ooi, T. et al. Cloning and sequencing of a Deleya marina gene encoding for alginate lyase. Biotechnology Letters 21, 169–174 (1999). https://doi.org/10.1023/A:1005435725903

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  • DOI: https://doi.org/10.1023/A:1005435725903

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