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Modification of hydrophilicity/hydrophobicity of the microenvironment of lipase of Candida rugosa by dextrans

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Abstract

Replacing the lactose used in the commercial preparation of lipase from Candida rugosa by dextrans with different molecular weight, several preparations with enhanced activities in esterification of (R,S)-ibuprofen in organic medium were obtained. The presence of carbohydrates modifies the microenvironment of the enzyme and maintains the hydration of the biocatalyst. We can modulate the hydrophilic/hydrophobic balance on the surface of the biocatalyst creating non covalent enzyme-dextran complexes.

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Authors and Affiliations

  1. Department of Organic and Pharmaceutical Chemistry, Complutense University, 28040, Madrid, Spain

    R.M. de la Casa, J.M. Sánchez-montero & J.V. Sinisterra

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  1. R.M. de la Casa
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  2. J.M. Sánchez-montero
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  3. J.V. Sinisterra
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de la Casa, R., Sánchez-montero, J. & Sinisterra, J. Modification of hydrophilicity/hydrophobicity of the microenvironment of lipase of Candida rugosa by dextrans. Biotechnology Letters 21, 123–128 (1999). https://doi.org/10.1023/A:1005426101080

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  • DOI: https://doi.org/10.1023/A:1005426101080

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