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Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum

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Abstract

A conserved region of a polygalacturonase (PG) gene from Penicillium griseoroseum was PCR amplified and used to screen a genomic library from this fungus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalacturonases. DNA hybridization analyses revealed that there is a low copy number of pggI in the P. griseoroseum genome, probably one or two copies.

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Authors and Affiliations

  1. Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    A.O.B. Ribon, J.L.C. Coelho & E.F. Araújo

  2. Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, 36571-000, Brazil

    E.G. de Barros

Authors
  1. A.O.B. Ribon
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  2. J.L.C. Coelho
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  3. E.G. de Barros
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  4. E.F. Araújo
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Ribon, A., Coelho, J., de Barros, E. et al. Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum . Biotechnology Letters 21, 395–399 (1999). https://doi.org/10.1023/A:1005419225412

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  • DOI: https://doi.org/10.1023/A:1005419225412

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