Abstract
A new thermophilic strain of Bacillus SPS-0 which produces thermostable xylanases was isolated from a hot spring in Portugal. Xylanase production was 50 nkat/ml in the presence of wheat bran arabinoxylan. The temperature and pH for optimum activity were 75°C and 6–9, respectively. The hydrolysis patterns demonstrated that crude xylanases yield mainly xylose and xylobiose from xylan, whereas xylose and arabinose were produced from destarched wheat bran. An increase in xylose release was observed when SPS-0 xylanase was supplemented by a ferulic acid esterase. © Rapid Science Ltd. 1998
Similar content being viewed by others
References
Bailey M.J., Biely P. and Poutanen K. (1992). J. Biotechnol. 23, 257–270.
Bartolomé B., Faulds C.B., Tuohy M., Hazlewood G.P., Gilbert H.J. and Willianson G. (1995). Biotechnol. Appl. Bioc. 22, 65–73.
Bataillon M., Mathali P., Cardinali A.P. and Duchiron F. (1998). Ind. Crops Prod. 8, 37–43.
Beldman G., Osuga D. and Whitaker J.R. (1996). J. Cereal Sci. 23, 169–180.
Bradford M.M. (1976). Anal. Biochem. 72, 248–254.
Dimitrov P.L., Kambourova M.S. Mandeva R.D. and Emanuilova E.I. (1997). FEMS Microbiol. Lett., 157, 27–30.
Faulds C.B. and Williamson G. (1994). Microbiology. 140, 779–787.
Gilead S. and Shoham Y. (1995). Appl. Environ. Microb. 61, 170–174.
Herr D., Baumer F. and Dellweg H. (1978). Appl. Microbiol. Biot. 5, 29–36.
López C., Blanco A. and Pastor J.F.I. (1998). Biotechnol. Lett., 20, 243–246.
Miller G. L. (1959). Anal. Chem., 31, 426–428.
Morales P., Madarro A., Flors A., Sendra J.M. and Pérez-González J.A. (1995). Enzyme Microb. Tech. 17, 424–429.
Naudin O. and De Baynast R. (1990). Procédé de préparation de son de blé désamylacé et produit obtenu. European Patent 90 401 461.0.
Subramaniyan S., Prema P. and Ramakrishna V. (1997). J. Basic Microb. 37, 431–437.
Sunna A., Prowe S.G., Stoffregen T. and Antranikian G. (1997). FEMS Microbiol. Lett. 148, 209–216.
Voragen A.G.J., Bergmans M.E.F, Oosterveld A., Schols H.A. and Beldman G. (1997). Carbohydr. Org. Raw Mater. III, Workshop. Van Bekkum H., Roeper H. and Voragen A.G.J. eds., p 1–16.
Weinstein L. and Albershein P. (1979). Plant. Physiol. 63, 425–432.
Williams R.A.D. and Da Costa M.S. (1992). The genus Thermus and related microorganisms. In: The Prokaryotes, New-York: Springer-Verlag, p. 3745–3753.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bataillon, M., Nunes Cardinali, AP. & Duchiron, F. Production of xylanases from a newly isolated alkalophilic thermophilic Bacillus sp.. Biotechnology Letters 20, 1067–1071 (1998). https://doi.org/10.1023/A:1005415207188
Issue Date:
DOI: https://doi.org/10.1023/A:1005415207188