Skip to main content
Log in

Ethanol production from dilute acid hydrolysate of rice hulls using genetically engineered Escherichia coli

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Approximately 30% of rice hulls, which represent an abundant residue with little commercial value, was solubilized with 0.4 M H2SO4 acid to produce a syrup containing over 100 g monomer sugar/l. Toxins generated during hydrolysis were mitigated with Ca(OH)2. Treated hydrolysate plus additional nutrients was fermented with Escherichia coli KO11 to produce over 46 g ethanol/L in 72 h (92% of theoretical yield). © Rapid Science Ltd. 1998

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Amartey, S and Jeffries, TW (1994). Biotechnol Lett 16:211–214

    Google Scholar 

  • Atlas, RM and Parks, LC (1993). Handbook of Microbiological Media, pp 529, Florida: CRC Press

    Google Scholar 

  • Beall, DS, Ohta, K and Ingram, LO (1991). Biotechnol Bioeng 38:296–303

    Google Scholar 

  • Buchert, J, Niemela, K, Puls, J and Poutanen, K (1990). Process Biochem Int 25:176–180

    Google Scholar 

  • du Preez, C (1994). Enzyme Microb Technol 16:944–956

    Google Scholar 

  • Frazer, FR and McCaskey, TA (1991). Enzyme Microb Technol 13:110–115

    Google Scholar 

  • Grohmann, K, Torget, R and Himmel, M (1985). Biotechnol Bioengin 15:59–80

    Google Scholar 

  • Hussein, AM, El-Saied, H and Yasin, MH (1992). Chem Tech Biotechnol 53:147–152

    Google Scholar 

  • Ingram, LO, Gomez, PF, Lai, X, Moniruzzaman, M, Wood, BE, Yomano, LP and York, SE (1998). Biotechnol Bioengin 58:204–214

    Google Scholar 

  • Ingram, LO, Lai, X, Moniruzzaman, M, Wood, BE and York, SE (1997). Amer Chem Soc Symp 666:57–73

    Google Scholar 

  • Juliano, BO (1985). Rice hulls and straw. In: Rice Chemistry and Technology, B.O. Juliano, ed, pp 689–743, Minnesota: The American Association of Cereal Chemists Incorporated

    Google Scholar 

  • McMillan, JD (1994). Amer Chem Soc Symp 566:411–437

    Google Scholar 

  • Ohta, K, Beall, DS, Mejia, JP, Shanmugam, KT and Ingram, LO (1991). Appl Environ Microbiol 57:893–900

    Google Scholar 

  • Olsson, L and Hahn-Hagerdal, B (1996). Enzyme Microb Technol 18:312–331

    Google Scholar 

  • Osborn, D and Chen, LF (1984). Starch/Starke 36:393–395

    Google Scholar 

  • Palmqvist, E (1998). Fermentation of lignocellulosic hydrolysates: Inhibition and detoxification. Doctoral dissertation. Lund University, Department of Applied Microbiology, Lund, Sweden.

    Google Scholar 

  • Robyt, JF (1998). Essentials of Carbohydrate Chemistry, pp 50–57, New York: Springer

    Google Scholar 

  • Saier, MH (1998). Biotechnol Bioengin 58:170–174

    Google Scholar 

  • Shah, MM, and Cheryan, M (1995). J Indust Microbiol 15:4214–4218

    Google Scholar 

  • Tagami, H and Aiba, H (1998). EMBO J 17:1759–1767

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moniruzzaman, M., Ingram, L.O. Ethanol production from dilute acid hydrolysate of rice hulls using genetically engineered Escherichia coli. Biotechnology Letters 20, 943–947 (1998). https://doi.org/10.1023/A:1005412120830

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005412120830

Navigation