Skip to main content
Log in

Stability of xylanases in the presence of methanol and its evaluation on the bleaching capacity

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A commercial (Cartazyme) and non-commercial (Asperzyme) xylanases were studied. Cartazyme stability in a 0–70% (v/v) methanol at 50 °C and 65 °C was carried out. No deactivation was found for Cartazyme in the presence of 15% methanol at 50 °C. Half-life activity decay (t1/2) of Cartazyme at 50 °C in 30%, 50% and 70% methanol solutions were 4.0 h, 2.3 h and 1.2 h, respectively. At 65 °C, which is the ozone-alkali-peroxide (ZEP) bleaching temperature, only significant results on Kappa number reduction and selectivity were only observed in 15% methanol (t1/2 30 min) at the Z stage. For the Asperzyme, a t1/2 of 36.5 min at 50 °C was found. In the Z stage with Asperzyme in the presence of 25% of methanol, a 20% Kappa number reduction and an improvement of the ZEP sequence of the brightness of 3.1 points were obtained. These results were correlated with the xylanase stability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Aguirre C, Ruiz J, Castillo C, Nazal A, Baeza J Durán N, Curotto E (1995) Proc. Fourth Brazilian Symp. on the Chem. Lignins and Other Wood Comp. 5: 170–183. Chem. Abstr. 126: 90902 (1997).

    Google Scholar 

  • Allison RW, Clark TA, Ellis MJ (1995) APPITA J. 48: 201–206.

    Google Scholar 

  • Angelo R, Aguirre C, Curotto E, Esposito E, Fontana JD, Baron M, Milagres AMF, Durán N (1997) Biotechnol. Appl. Biochem. 25: 19–27.

    Google Scholar 

  • Angelo RS, Durán N (1998a) Proc. 7th Int. Conf. Biotechnol. Pulp Paper Ind. Vancouver, BC, Canada, C19–C22.

  • Angelo RS, Durán N (1998b) Proc. 5th Eur. Workshop Lignocell. and Pulp Advances in Lignocellulosics Chem. Ecol. Friendly Pulping Bleaching Technol. Aveiro, Portugal, pp. 505–508.

  • Baeza J, Freer J, Grandon J, Ruiz J, Rodriguez J, Curotto E, Durán N (1999) Proc. 10th Intern. Symp. Wood and Pulping Chem., Yokohama, Japan, in press.

  • Bailey MJ, Biely P, Poutanen K (1992) J. Biotechnol. 23: 257–270.

    Google Scholar 

  • Bakalova N, Petrova S, Benadova R, Kolev D (1995) Biotechnol. Equip. 2: 45–51.

    Google Scholar 

  • Curotto E, Aguirre C, Concha M, Nazal A, Campos V, Esposito E. Angelo R, Milagres AMF, Durán N (1993) Biotechnol. Tech. 7: 821–822.

    Google Scholar 

  • Curotto E, Nazal A, Aguirre C, Campos V, Durán N (1998) Appl. Biochem. Biotechnol. 73: 29–42.

    Google Scholar 

  • Durán N, Angelo R (1998) Proc. Fifth Workshop on Lignocellulosics and Pulp-EWLP-98. Aveiro, Portugal, pp. 505–508.

  • Durán N, Curotto E, Esposito E, Aguirre C, Nazal A, Angelo R (1994) In: Galindo E, Ramirez OT, eds. Advances in Bioprocesses Engineering. Kluwer Academic Publishers, Dordrecht, pp. 489–494.

    Google Scholar 

  • Eriksson K-EL, ed. (1997) Biotechnology in the pulp and paper industry. In: Advances in Biochemical Engineering Biotechnology, Vol. 7.

  • Ganga A, Gonzalez-Candelas L, Ramon D, Perez-Gonzalez JA (1997) J. Agric. Food Chem. 45: 2359–2362.

    Google Scholar 

  • Gupta MN, Batra R, Tyagi R, Sharma A (1997) Biotechnol. Prog. 13: 284–288.

    Google Scholar 

  • Herrmann M, Kubicek CP (1996) In: Srebotnik E, Messner K, eds. Biotechnology in the Pulp and Paper industry: Recent Advances in Applied and Fundamental Research. Facultas-Universitatsverlag, Austria, pp. 103–106.

    Google Scholar 

  • Kibblewhite RP, Clark TA (1996) APPITA J. 49: 390–396.

    Google Scholar 

  • Prade RA (1996) Biotechnol. Genet. Eng. Rev. 13: 101–131.

    Google Scholar 

  • Ruiz J, Baeza J, Freer J, Curotto E, Esposito E, Durán N (1993) Proc. Third Braz. Symp. Chem. Lignins and Other Wood Comp. 4: 324–327. Chem. Abstr. 124: 120498 (1993).

    Google Scholar 

  • Ruiz J, Freer J, Rodriguez J, Baeza J (1997) Wood Sci. Technol. 31: 217–223.

    Google Scholar 

  • Srebotnik E, Messner K, eds. (1996) Biotechnology in the Pulp and Paper Industry: Recent Advances in Applied and Fundamental Research. Facultas-Universitatsverlag, Austria.

    Google Scholar 

  • Sung C-K, Lee S-W, Park S-K, Chun S-S (1996) Han'guk Sikp'um Yongyang Kwahak Hoechi 25: 701–707.

    Google Scholar 

  • Tan SKH, Omar O, Kader JA (1993) Proc. 18th. Malaysia Biochem. Soc. Conf.: 74–80.

  • Vicuña R, Yeber MC, Osses M (1995) J. Biotechnol. 42: 69–73.

    Google Scholar 

  • Viikari L, Buchert J, Suurnäkki A (1997) In: Bruce A, Palfreyman JW, eds. Forest Products Biotechnology. New York: Taylor & Francis Publ., pp. 83–116.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ruiz, J., Angelo, R., Freer, J. et al. Stability of xylanases in the presence of methanol and its evaluation on the bleaching capacity. Biotechnology Letters 21, 361–366 (1999). https://doi.org/10.1023/A:1005403126642

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005403126642

Navigation