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High level secretion of recombinant staphylokinase into periplasm of Escherichia coli

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Abstract

The staphylokinase (SAK) gene from Staphylococcus aureus NCTC10033 was inserted into an expression vector, pKK-ompA, having a tac promoter and an ompA signal sequence. Escherichia coli JM109 carrying the recombinant plasmid produced and secreted the recombinant SAK (rSAK) at 15ug/ml into periplasm and 5ug/ml to extracellular media, respectively. The rSAK was purified with 59% yield by simple procedures from the periplasm of E. coli. The amino-terminal sequence and human plasminogen activating activity of rSAK were coincided with the authentic SAK.

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References

  • Behnke, D and Gerlach, D (1987). Mol Gen Genet 210: 528-534

    Google Scholar 

  • Collen, D, De-Cock, F and Stassen, JM (1993). Circulation 87: 996-1006

    Google Scholar 

  • Collen, D, Silence, K, Demarsin, E, De Mol, M and Lijnen, HR (1992). Fibrinolysis 6: 203-213

    Google Scholar 

  • Ko, JH, Park, DK, Kim, IC, Lee, SH and Byun, SM (1995). Biotechnology Letters 17: 1019-1024.

    Google Scholar 

  • Lack, CH (1948). Nature 161: 559-560

    Google Scholar 

  • Lijnen, HR, Van-Hoef, B, De-Cock, F, Okada, K, Ueshima, S, Matsuo, O and Collen, D (1991). J Biol Chem 266: 11826-11832

    Google Scholar 

  • Sakai, M, Watanuki, M and Matsuo, O (1989). Biochem Biophys Res Comm 162: 830-837

    Google Scholar 

  • Sako, T, Sawaki, S, Sakurai, T, Ito, S, Yoshizawa, Y and Kondo, I (1983). Mol Gen Genet 190: 271-277

    Google Scholar 

  • Schlott, B, Hartmann, M, G(81)hrs, K, Birch-Hirschfeid, E, Pohl, H, Vanderschueren, S, Van der Werf, F, Michoel, A, Collen, D and Behnke, D (1994). Bio/technology 12: 185-189

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, KAIST (Korea Advanced Institute of Science and Technology), and RCNBMA (Research Center for New Bio-Materials in Agriculture), 373-1, Kusong-dong, Yousong-gu Taejon, Korea

    Sang Jun Lee, Il Chul Kim, Dong Min Kim, Kwang Hee Bae & Si Myung Byun

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  1. Sang Jun Lee
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  2. Il Chul Kim
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  3. Dong Min Kim
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  4. Kwang Hee Bae
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  5. Si Myung Byun
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Lee, S.J., Kim, I.C., Kim, D.M. et al. High level secretion of recombinant staphylokinase into periplasm of Escherichia coli. Biotechnology Letters 20, 113–116 (1998). https://doi.org/10.1023/A:1005359920522

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