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Production in sea-water of thermostable alkaline proteases by a halotolerant strain of Bacillus licheniformis

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A halotolerant strain of Bacillus licheniformis, previously isolated from marine sediments, produced high protease activity during the early stationary phase of growth. The use of sea-water in the fermentation medium enhanced the production of this activity of 150%. After a partial purification, three different proteolytic enzymes could be detected, which were alkaline serine proteases, exhibiting optimal activity at pH 9.0 and at 70°C. The proteases were activated by NaCl, with a two, three-fold increase in activity and were stable in presence of 0.7% NaBO3, 0.5% NaClO and 3% H2 O2. © Rapid Science Ltd. 1998

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Manachini, P., Fortina, M. Production in sea-water of thermostable alkaline proteases by a halotolerant strain of Bacillus licheniformis. Biotechnology Letters 20, 565–568 (1998).

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