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Production in sea-water of thermostable alkaline proteases by a halotolerant strain of Bacillus licheniformis

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Abstract

A halotolerant strain of Bacillus licheniformis, previously isolated from marine sediments, produced high protease activity during the early stationary phase of growth. The use of sea-water in the fermentation medium enhanced the production of this activity of 150%. After a partial purification, three different proteolytic enzymes could be detected, which were alkaline serine proteases, exhibiting optimal activity at pH 9.0 and at 70°C. The proteases were activated by NaCl, with a two, three-fold increase in activity and were stable in presence of 0.7% NaBO3, 0.5% NaClO and 3% H2 O2. © Rapid Science Ltd. 1998

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References

  • Andrews, P (1964). Biochem. J. 91: 222–232

    PubMed  Google Scholar 

  • Bradford, MM (1976). Anal. Biochem. 72: 248–254

    Article  PubMed  Google Scholar 

  • Galinski, EA and Tindall, BJ (1992). Biotechnological prospects for halophiles and halotolerant microorganisms. In: Molecular Biology and Biotechnology of Extremophiles, RA Herbert and RJ Sharp, eds, pp 76–114, London: Blackie

    Google Scholar 

  • Gerhartz, W (ed) (1990). Industrial use of enzymes. In: Enzymes in Industry. Production and Applications, pp 77–149, Weinheim: VCH

  • Greene, RV, Griffin, HL and Cotta, MA (1996). Biotechnol. Lett. 18: 759–764

    Google Scholar 

  • Kirk, RE and Othmer, DE (1980). Enzyme detergents and industrial enzymes. In: Kirk-Ozhzaer Enclyclopedia of Chemical Technology, vol.9 pp 138–224, New York: Wiley & Sons

    Google Scholar 

  • Manachini, PL and Fortina, MG (1994). Folia Microbiol. 39: 378–380

    Google Scholar 

  • Manachini, PL, Fortina, MG and Parini, C (1988). Appl. Microbiol. Biotechnol. 28: 409–413

    Google Scholar 

  • Morihara, K (1974). Adv. Enzymol. 41: 179–243

    PubMed  Google Scholar 

  • Strongin, AYA, Izotova, LS, Abramov, ZT, Gorodetsky, DI, Ermakova, LM, Baratova, LA, Belyanova, LP and Stepanov, VM (1978). J. Bacteriol. 133: 1401–1411

    PubMed  Google Scholar 

  • Zukowski, MM (1992). Production of commercially valuable products. In: Biology of Bacilli: Applications to Industry, RH Doi and M McGloughlin, eds, pp 311–337 London: Butterworth-Heinemann

    Google Scholar 

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Manachini, P., Fortina, M. Production in sea-water of thermostable alkaline proteases by a halotolerant strain of Bacillus licheniformis. Biotechnology Letters 20, 565–568 (1998). https://doi.org/10.1023/A:1005349728182

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