Abstract
A medium containing cornsteep liquor (CSL) as a replacement for yeast extract (YE) enhanced the sporulation of Bacillus sphaericus 2362. Fed-batch culture with combinations of YE and CSL together with acetate as carbon source resulted in a cell mass of 13.0 g/L and a high spore count of 16.4×109/mL. This compared to an acetate, YE based medium which produced a similar cell concentration but only 4×109 spores/mL. Western blot analysis indicated significant toxin production in the former case.
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References
Arcas, J., Yantorno, E., Arcas, E., and Ertola, R. (1984). Biotechnol. Lett. 8, 495-500
Broadwell, A.H., Baumann, L., and Baumann, P. (1990). J. Bacteriol. 172, 2217-2223
Carlsen, F., and Emborg, C. (1981). Biotechnol. Lett. 3, 375-378
Davidson, E.W., and Myers, P. (1981). FEMS Microbiol.Lett. 10, 261-265
Davidson, E.W., Urbina, M., Payne, J., Mulla, M.S., Darwazen, H., Dulmage, H.T., and Correa, J.A. (1984). Appl. Environ. Microbiol. 47, 125-129
Elcin, Y.M. (1995). Enzyme Microb. Technol., 17, 587-591
Gangurde, R.P., and Shethna, Y.I. (1995). World J. Microbiol. Biotechnol., 11, 202-205
Jong, J.Z., Wu, W.T., and Tzeng, Y.M. (1994). Biotechnol. Tech., 8, 483-486
Kang, B.C., Lee, S.Y., and Chang, H.N. (1992). Biotechnol. Lett. 14, 721-726
Kang, B.C., Lee, S.Y., and Chang, H.N. (1993). Biotechnol. Bioeng. 42, 1107-1112 (1993)
Karim, M.I.A., Lucas, R.J., Osborne, K.J. and Rogers, P.L. (1993). Biotechnol.Lett., 15, 47-50
Mitani, T., Heinze, J.E., Freese, E. (1977). Biochem. Biophys. Res. Commun. 77, 1118-1125
Osborne, K.J. (1995). PhD thesis, University of New South Wales, Australia, 51-57
Pearson, D., and Ward, O.P. (1988). Biotechnol. Lett. 7, 451-456
Priest, F.G. (1992). J.Appl.Bacteriol. 72, 357-369
Sasaki, K., Idachaba, M.A., and Rogers, P.L. (1995). Biotechnol. Tech. 9, 321-326
Thanabalu, T., Hindley, J., Jackson-Yap, J., and Berry, C. (1991). J. Bacteriol. 173, 2776-2785
Yousten, A.A., and Wallis, D.A. (1987). J. Ind. Microbiol. 2, 277-283
Yousten, A.A., Wallis, D.A., and Singer, S. (1984). Curr. Microbiol. 11, 175-178
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Sasaki, K., Jiaviriyaboonya, S. & Rogers, P. Enhancement of sporulation and crystal toxin production by cornsteep liquor feeding during intermittent fed-batch culture of Bacillus sphaericus2362. Biotechnology Letters 20, 165–168 (1998). https://doi.org/10.1023/A:1005332609136
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DOI: https://doi.org/10.1023/A:1005332609136