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Optimization of dilution rate, pH and oxygen supply on optical purity of 2, 3-butanediol produced by Paenibacillus polymyxa in chemostat culture

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Abstract

The optimum dilution rate for 2, 3-butanediol (BDL) production by Paenibacillus polymyxa was 0.2 h1 and the optical purity of BDL remained above 98 % at all dilution rates. With decreasing culture pH, ethanol and BDL production increased, whereas the optical purity of BDL decreased to 94 % at pH 5.7. In the chemostat culture at pH 6.3 and a 0.1 h1 dilution rate, the optimum air supply for BDL production was 200 ml min−1 in which the O2 uptake rate was 6.7 mmol l−1 h−1. Under this condition, the optical purity of BDL decreased to 93 %. © Rapid Science Ltd. 1998

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References

  • deMas, C, Jansen, NB and Tsao, GT (1987). Biotechnol Bioeng 31: 366–377

    Google Scholar 

  • Jansen, NB, Flickinger, MC and Tsao, GT (1984). Biotechnol Bioeng 26: 362–369

    Google Scholar 

  • Haughney, H and Nauman, EB (1990). Biotechnol Bioeng 36: 142–248

    Google Scholar 

  • Mankad, T and Nauman, EB (1992). Biotechnol Bioeng 40: 413–426

    Google Scholar 

  • Mickelson, M and Werkman, CH (1938). J Bacteriol 36: 67–76

    Google Scholar 

  • Miller, TL and Wolin, MJ (1974). Appl Microbiol 27: 985–987

    Google Scholar 

  • Ramos, A, Jordan, KN, Cogan, TM and Santos, H (1994). Appl Environ Microbiol 60: 1739–1748

    Google Scholar 

  • Ui, S, Masuda, H and Muraki, H (1983). J Ferment Technol 61: 253–259

    Google Scholar 

  • Ui, S, Matsuda, T, Masuda, H and Muraki, H (1986). J Ferment Technol 64: 481–486

    Google Scholar 

  • Zeng, A-P, Biebl, H and Deckwer, W-D (1990 a). Appl Microbiol Biotechnol 33: 264–268

    Google Scholar 

  • Zeng, A-P, Biebl, H and Deckwer, W-D (1990 b). Appl Microbiol Biotechnol 33: 485–489

    Google Scholar 

  • Zeng, A-P, Byun, T-G, Posten, C and Deckwer, W-D (1994). Biotechnol Bioeng 44: 1107–1114

    Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Kagamiyama, Hiroshima, Japan

    Y. Nakashimada, K. Kanai & N. Nishio

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  1. Y. Nakashimada
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  2. K. Kanai
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  3. N. Nishio
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Nakashimada, Y., Kanai, K. & Nishio, N. Optimization of dilution rate, pH and oxygen supply on optical purity of 2, 3-butanediol produced by Paenibacillus polymyxa in chemostat culture. Biotechnology Letters 20, 1133–1138 (1998). https://doi.org/10.1023/A:1005324403186

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