Abstract
Growth of Zymomonas mobilis ZM4 in media containing sodium acetate was inhibited above 12 g sodium acetate/l at pH 5.0. Following mutagenesis of ZM4, an acetate-tolerant strain was isolated in continuous culture that grew in the presence of 20 g sodium acetate/l at pH 5.0. In continuous culture with complete cell recycle at 30 deg C and pH 5.4 using media containing 110 g glucose/l, the maintenance energy coefficient (m) for the mutant was found to increase from 1.9 g glucose/g cell dry wt.h at 12 g sodium acetate/l up to 3.9 g/g.h at 20 g/l.
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Joachimsthal, E., Haggett, K.D., Jang, JH. et al. A mutant of Zymomonas mobilis ZM4 capable of ethanol production from glucose in the presence of high acetate concentrations. Biotechnology Letters 20, 137–142 (1998). https://doi.org/10.1023/A:1005320306410
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DOI: https://doi.org/10.1023/A:1005320306410