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A new device for measuring diffusional restrictions and modelling substrate inhibition in a starch-glucoamylase system

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Abstract

An experimental device that quantifies the diffusional properties of polysaccharides solution, in a simple and reproducible way, and an equation relating these properties to solute concentration, is proposed. Using this model to analyse the kinetic profile of the hydrolysis of starch with glucoamylase, is substrate inhibition completely reduced to phenomena that depends on the reological properties of the reacting mixture. The experimental device and kinetic model can be applied to contrast hypothetical substrate inhibitions in any enzymatic system.

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Authors and Affiliations

  1. Departamento de Bioquimica, Universidade de Vigo, As Lagoas Ourense, Spain

    L. Pastrana

  2. Instituto de Investigacions Marinas de Vigo (CSIC), r/ Eduardo Cabello, Vigo, Spain

    MaP. Gonzalez, J. Miron & M.A. Murado

Authors
  1. L. Pastrana
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  2. MaP. Gonzalez
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  3. J. Miron
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  4. M.A. Murado
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Pastrana, L., Gonzalez, M., Miron, J. et al. A new device for measuring diffusional restrictions and modelling substrate inhibition in a starch-glucoamylase system. Biotechnology Letters 20, 127–130 (1998). https://doi.org/10.1023/A:1005316205501

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  • DOI: https://doi.org/10.1023/A:1005316205501

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