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Magnetic sorting in plant tissue: immunoseparation of pea nuclei with an antibody against nucleoporin

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Abstract

Nuclei were magnetically separated from root extracts of Pisum sativum L. by means of a MiniMACS separation unit after indirect labelling with the antibody mAb414 (raised against rat liver nucleoporin p62) and a secondary anti-mouse antibody labelled with superparamagnetic MACS Microbeads.

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Authors and Affiliations

  1. Istituto Policattedra - Biotecnologie Agro-Industriali, Universita di Verona, Strada le Grazie Ca Vignal, Verona, Italy

    Paola Malacrino, Flavia Guzzo & Marisa Levi

  2. Servizio di Anatomia Patologica, Azienda Ospedaliera Ospedale Niguarda Ca Granda, Milano, Italy

    Stefania Grignani

Authors
  1. Paola Malacrino
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  2. Stefania Grignani
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  3. Flavia Guzzo
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  4. Marisa Levi
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Malacrino, P., Grignani, S., Guzzo, F. et al. Magnetic sorting in plant tissue: immunoseparation of pea nuclei with an antibody against nucleoporin. Biotechnology Letters 20, 117–121 (1998). https://doi.org/10.1023/A:1005312004593

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  • DOI: https://doi.org/10.1023/A:1005312004593

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