Abstract
Production of poly (3-hydroxybutyrate) (PHB) from starch was investigated in flask, batch, and fed-batch cultures of Azotobacter chroococcum. In flask culture, PHB content increased up to 74% of dry cell wt with increasing culture volume. In batch culture, PHB content increased to 44% with O2 limitation. In fed-batch culture, cell concentration of 71 g/l with 20% PHB was obtained without O2 limitation, whereas cell concentration of 54 g/l with 46% PHB was obtained with O2 limitation.
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References
Buchanan, RE and Gibbons, NE (1974). Bergey's Manual of Determinative Bacteriology, 8th ed, p 754, The Williams & Wilkins Company
Braunegg, G, Sonnleitner, B and Lafferty, RM (1978). Eur J Appl Microbiol Biotechnol 6:29-37
Guimaraes, WV, Ohta, K, Burchhardt, G and Ingram, LO (1992). Biotechnol Lett 14:415-420
Hahn, SK, Chang, YK, Kim, BS and Chang, HN (1994). Biotechnol Bioeng 44:256-261
Hughes, L and Richardson, KR (1984). US Patent 4,433,053
Kim, BS and Chang, HN (1995). Biotechnol Technique 9:311- 314
Kim, BS, Lee, SC, Lee, SY, Chang, HN, Chang, YK and Woo, SI (1994). Biotechnol Bioeng 43:892-898
Lee, IY, Stegantseva, EM, Savenkova, L and Park, YH (1995). J Microbiol Biotechnol 5:100-104
Lee, SY (1996). Biotechnol Bioeng 49:1-14
Page, WJ (1989). Appl Microbiol Biotechnol 31:329-333
Page, WJ and Knosp, O (1987). Appl Env Microbiol 55:1334-1339
Rodriguez-Valera, F and Lillo, JAG (1992). FEMS Microbiol Rev 103:181-186
Senior, PJ, Beech, GA, Ritchie, GAF and Dawes, EA (1972). Biochem J 128:1193-1201
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Kim, B.S., Chang, H.N. Production of poly (3-hydroxybutyrate) from starch by Azotobacter chroococcum. Biotechnology Letters 20, 109–112 (1998). https://doi.org/10.1023/A:1005307903684
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DOI: https://doi.org/10.1023/A:1005307903684