Abstract
Thirty-two mature crossbred (Fine Wool Synthetic, FWS) sheep developed for fine wool production in India were treated for superovulation and oestrus synchronization in spring season. The ewes were randomly allocated to four treatment groups in a factorially designed experiment. For induction of superovulation, pregnant mare serum gonadotrophin (PMSG) was administered alone (group 1), in combination with gonadotrophin releasing hormone (GnRH) (group 2) or with follicle stimulating hormone (FSH) (group 3) and with both (group 4). Oestrus was synchronized in all the ewes by two injections of prostaglandin F2α (PGF, 10 mg each) administered at an interval of 10 days. Superovulation treatment started 48 h prior to the second PGF injection. The proportion of ewes in oestrus did not differ significantly (p>0.05) in the four groups. The use of GnRH set the ewes into oestrus earlier than the ewes in the other groups. Treatment with PMSG (800 IU) in conjunction with 4 μg of Buserilin (GnRH) increased the ovulation rate (9.1±2.6 corpora lutea (CL)) above that observed when PMSG was used alone (3.0±0.7 CL). The use of FSH (0.5 U ovagen) in conjunction with PMSG was characterized by a decrease in the proportion of ewes with ≤2 CL (4/8 vs 7/8; p<0.05) and in the number of ovulations, i.e. CL observed (2.4±0.6 vs 9.1±2.6), and a nonsignificant increase in the incidence of large follicles (LF) (4.6±1.28 vs 3.25±0.6; p>0.05). The interaction between treatments of FSH and GnRH was not significant (p>0.05).
It is concluded that use of GnRH, in conjunction with either PMSG alone or PMSG plus FSH treatment, advanced the onset of oestrus and increased the ovulation rate in FWS sheep.
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REFERENCES
Boland, M.P., O'Doherty, J.V. and Crosby, T.F., 1993. Superovulation in sheep influenced by FSH and body condition. Proceedings of the 9th Meeting of the European Embryo Transfer Association, Lyon, p. 158
Cseh, S. and Seregi, J., 1993. Practical experiences with sheep embryo transfer. Theriogenology, 39, 207
Cumming, F.A., Brown, J.M., Blockey, M.A., Winfield, C.G., Baxter, R. and Goding, I.R., 1971. Consistency of interval between luteinizing hormone release and ovulation in the ewe. Journal of Reproduction and Fertility, 24, 134-135
Gordon, I., 1997. Embryo transfer and associated techniques in sheep. In: Controlled Reproduction in Sheep and Goat, (CAB International, Wallingford, Oxon)
Hafez, E.S.E., 1987. Embryo transfer, IVF and genetic engineering. In: E.S.E. Hafez (ed.), Reproduction in Farm Animals, 5th edn, (Lea and Febiger, Philadelphia), 528-570
Harvey, W.A., 1990. Mixed mode least square and maximum likehood computer programme.
Jabbour, H.N. and Evans, G., 1991a. Ovarian and endocrine responses of Merino ewes to treatment with PMSG and/or FSH-P. Animal Reproduction Science, 26, 93-106
Jabbour, H.N. and Evans, G., 1991b. Ovarian and endocrine responses of Merino ewes following treatment with PMSG and GnRH or PMSG antiserum. Animal Reproduction Science, 24, 259-270
Jabbour, H.N., Ryan, J.P., Evans, G. and Maxwell, W.M.C., 1991. Effect of season, GnRH administration and lupin supplementation on the ovarian and endocrine response of Merino ewes treated with PMSG and FSH-P to induce superovulation. Reproduction, Fertility and Development, 3, 699-707
Mutiga, E.R. and Baker, A.A., 1982. Ovarian response, ova recovery and fertility in Merino ewes superovulated either during luteal phase of their estrus cycle or after intravaginal progestagen treatment. Theriogenology, 17, 537-544
Nancarrow, C.D., Murray, J.D., Boland, M.P., Sutton, R. and Hazelton, I.G., 1984. Effect of gonadotrophin releasing hormone in production of single cell embryos for pronuclear injection of foreign genes. In: D.R. Lindray and D.T. Pearce (eds), Reproduction in Sheep, (Australian Academy of Science, Canberra), 286-288
Ryan, J.P., Hunton, J.R. and Maxwell, W.M.C., 1991. Increased production of sheep embryos following superovulation of Merino ewes with a combination of pregnant mare serum gonadotrophin and follicle stimulating hormone. Reproduction, Fertility and Development, 3, 551-560
Ryan, J.P., Hunton, J.R. and Maxwell, W.M.C., 1992. Time of ovulation in Merino ewes superovulated with PMSG and FSH-P. Reproduction, Fertility and Development, 4, 91-97
Smith, C.L., 1984. Dose effect of follicle stimulating hormone for superovulation of crossbred Targhee ewes. Theriogenology, 21, 262 (abstract)
Smith, C., 1986. Use of embryo transfer in genetic improvement of sheep. Animal Production, 42, 81-87
Walker, S.K., Smith, D.H. and Seamark, R.F., 1986. Timing of multiple ovulation in the ewe after treatment with FSH or PMSG with and without GnRH. Journal of Reproduction and Fertility, 77, 135-142
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Naqvi, S., Gulyani, R. The Effect of Gonadotrophin Releasing Hormone and Follicle Stimulating Hormone in Conjunction with Pregnant Mare Serum Gonadotrophin on the Superovulatory Response in Crossbred Sheep In India. Tropical Animal Health and Production 30, 369–376 (1998). https://doi.org/10.1023/A:1005196705369
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DOI: https://doi.org/10.1023/A:1005196705369