Abstract
Seed bacterization with five plant growth promoting fluorescent Pseudomonas strains isolated from Indian and Swedish soils and three Rhizobium leguminosarumbiovar viceae strains isolated from Swedish soils were shown to promote plant growth in Pisum sativum L. cv. Capella. Co-inoculation of the fluorescent pseudomonads and Rhizobium improved plant growth in terms of shoot height, root length and dry weight. Both the fluorescent pseudomonads and Rhizobium were shown to exhibit a wide range of antifungal activity against pathogens specific to pea. Seed bacterization with plant growth promoting strains alone and together with a rhizobial isolate, R 361-27 reduced the number of infected peas grown in Fusarium oxysporum infested soils. We found that the introduced organisms were able to colonize the roots, which was confirmed using immunofluorescence staining and drug resistant mutant strains. In a synthetic culture medium, all the plant growth promoting fluorescent pseudomonads strains produced siderophores, which shown to express antifungal and antibacterial activity. Our results suggest the potential use of these bacteria to induce plant growth and disease suppression in sustainable agriculture production systems.
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Bezbaruah B, Dileep Kumar B S and Winkelmann G 1996 Influence of iron and antibiotic effect produced by rhizobacteria from tea (Camellia sinensis) plantations, Indian Pytopath. 49, 332–338.
Betelho G R, Guimaraes V, De Bonis M, Fonseca M E F, Hagler A N and Hagler L C M 1998 Ecology of a plant growth promoting strain of Pseudomonas fluorescens colonizing the maize endorhizosphere in tropical soil. World J. Microbiol. Biotechnol. 14, 499–504.
Chanway C P, Hynes R K and Nelson L M 1989 Plant growthpromoting rhizobacteria: Effects on growth and nitrogen of lentil (Lens esculenta Moench) and pea (Pisum sativum L.) Soil Biol. Biochem. 21, 511–517.
Dashti N, Zhang F, Hynes R and Smith D L 1997 Application of plant growth promoting rhizobacteria to soybean (Glycine max (L.) Merr.) increases protein and dry matter yield under shortseason conditions. Plant Soil 188, 33–41.
Dashti N, Zhang F, Hynes R and Smith D L 1998 Plant growth promoting rhizobacteria accelerate nodulation and increase nitrogen fixation activity by field grown soybean (Glycine max (L.) Merr.) under short seasons conditions. Plant Soil 200, 205–213.
Dileep Kumar B S 1999 Fusarial wilt suppression and crop improvement through two rhizobacterial strains in chick pea growing in soils infested with Fusarium oxysporum f. sp ciceris. Biol. Fert. Soils 29, 87–91.
Dileep Kumar B S and Dube H C 1992 Seed bacterization with a fluorescent Pseudomonas for enhanced plant growth, yield and disease control. Soil Biol Biochem. 24, 539–542.
Dowling D N and O' Gara F 1994. Metabolites of Pseudomonas involved in the biocontrol of plant disease. Tibtechnology 12, 133–141.
Duijff B J, Meijer J W, Bakker P A H M and Schippers B 1993 Siderophore mediated competition for iron and induced disease resistance in the suppression of Fusarium wilt of carnation by fluorescent Pseudomonas spp. Neth. J. Plant Pathol. 99, 277–291.
Elad Y and Baker R 1985a Influence of trace amounts of cations and siderophores producing pseudomonads on chlamydospore germination of Fusarium oxysporum. Phytopathology 75, 1047–1052.
Elad Y and Baker R 1985b The role of competition for iron and carbon in suppression of chlamydospore germination of Fusarium spp. by Pseudomonas spp. Phytopathology 75, 1053–1059.
Grims H D and Mount M S 1984 Influence of Pseudomonas putida on nodulation of Phaseolus vulgaris. Soil Biol. Biochem. 16, 27–30.
Lemenceau P, Bakker P A H M, De Kogel W J, Alabouvette C and Schippers B 1992 Effect of pseudobactin 358 production by Pseudomonas putida WCS 358 on suppression of Fusarium oxysporum FO47. Appl. Environ. Microbiol. 58, 2978–2982.
Li M and Alexander M 1988 Co-inoculation with antibiotic producing bacteria to increase colonization and nodulation by rhizobia. Plant Soil 108, 211–219.
Parmar N and Dadarwal K R 1999 Stimulation of nitrogen fixation and induction of flavonoid like compounds by rhizobacteria. J. Appl. Microbiol. 86, 36–44.
Podile A R 1995 Seed bacterization with Bacillus subtilis AF 1 enhances seedling emergence, growth and nodulation in pigeon pea. Ind. J. Microbiol. 35, 199–204.
Polonenko D R, Scher F, Kloepper J W, Singleton C A, Laliberate M and Zaleska I 1987 Effects of root colonizing bacteria on nodulation of soybean roots by Bradyrhizobium japonicum. Can. J. Microbiol. 33, 498–503.
Scher F M and Baker R 1980 Mechanisms of biological control in a Fusarium suppressive soil. Phytopathology 75, 412–417.
Scher F M and Baker R 1982 Effect of Pseudomonas putida and a synthetic iron chelator on induction of soil suppressiveness to Fusarium wilt pathogens. Phytopathology. 72, 1567–1573.
Schippers B 1993 Exploitation of microbial mechanisms to promote plant health and plant growth. Phytoparasitica 21, 275–279.
Sneh B, Dupler M, Elad Y and Baker R 1984 Chlamydospore germination of Fusarium oxysporum f. sp. cucmerinum as affected by fluorescent and lytic bacteria from Fusarium suppressive soils. Phytopathology 74, 1115–1124.
Somasegaran P and Hoben H J 1994 Handbook for Rhizobia: Methods in Legume-Rhizobium technology. pp 89–101. Springer Verlag New York Berlin Heidelberg
Thomashow L S and Macrodi D V 1997 The genetics and regulation of antibiotic production by PGPR. In Plant Growth Promoting Rhizobacteria - Present and Future Prospects. Eds. O. Ogoshi, K. Kobayashi, Y Homma, F Kodama, N Kondo and S Akino. pp 108–115. Proceedings of the Fourth International Workshop on Plant Growth-Promoting Rhizobacteria. Sapporo, Japan, October 5-10, 1997.
Van Peer R, Van Kuik A J, Rattink H and Schippers B 1990 Control of Fusarium wilt in carnation grown on rockwool by Pseudomonas sp. strain WCS 417r and by Fe-EDDHA. Neth. J. Plant Pathol. 96, 119–132.
Van Vuurde J W L and Van der Wolf J 1995 Immuno-fluorescence colony-staining (IFC). Molecular Microbial Ecology Manual 4.1.3, 1-19. IPO/DLO Wageningen, Kluwer Academic Publishers, Wageningen, the Netherlands.
Weller D M 1988 Biological control of soil borne plant pathogens in the rhizosphere with bacteria. Annu. Rev. Phytopathology 26, 379–407.
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Dileep Kumar, B.S., Berggren, I. & Mårtensson, A.M. Potential for improving pea production by co-inoculation with fluorescent Pseudomonas and Rhizobium. Plant and Soil 229, 25–34 (2001). https://doi.org/10.1023/A:1004896118286
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DOI: https://doi.org/10.1023/A:1004896118286