Effect of enriched rhizosphere carbon dioxide on nitrate and ammonium uptake in hydroponically grown tomato plants

Abstract

Previous reports have indicated positive effects of enriched rhizosphere dissolved inorganic carbon on the growth of salinity-stressed tomato (Lycopersicon esculentum L. Mill. cv. F144) plants. In the present work we tested whether a supply of CO₂ enriched air to the roots of hydroponically grown tomato plants had an effect on nitrogen uptake in these plants. Uptake was followed over periods of 6 to 12 hours and measured as the depletion of nitrogen from the nutrient solution aerated with either ambient or CO₂ enriched air. Enriched rhizosphere CO₂ treatments (5000 μmol mol^-1) increased NO₃ ^- uptake up to 30% at pH 5.8 in hydroponically grown tomato plants compared to control treatments aerated with ambient CO₂ (360 μmol mol^-1). Enriched rhizosphere CO₂ treatments had no effect on NH₃ ⁺ uptake. Acetazolamide, an inhibitor of apoplastic carbonic anhydrase, increased NO₃ ^- uptake in ambient rhizosphere CO₂ treatments, but had no effect on NO₃ ^- uptake in enriched rhizosphere CO₂ treatments. Ethoxyzolamide, an inhibitor of both cytoplasmic and extracellular carbonic anhydrase, decreased NO₃ ^- uptake in ambient rhizosphere CO₂ treatments. In contrast, a CO₂ enriched rhizosphere increased NO₃ ^- uptake with ethoxyzolamide, although not to the same extent as in plants without ethoxyzolamide. It is suggested that a supply of enriched CO₂ to the rhizosphere influenced NO₃ ^- uptake through the formation of increased amounts of HCO₃ ^- in the cytosol.