Plant and Soil

, Volume 194, Issue 1–2, pp 37–44 | Cite as

Association of nitrogen-fixing, plant-growth-promoting rhizobacteria (PGPR) with kallar grass and rice

  • K.A. Malik
  • Rakhshanda Bilal
  • Samina Mehnaz
  • G. Rasul
  • M.S. Mirza
  • S. Ali


Leptochloa fusca (L.) Kunth (kallar grass) has previously been found to exhibit high rates of nitrogen fixation. A series of experiments to determine the level of biological nitrogen fixation using N isotopic dilution were carried out in nutrient solution and saline soil. These studies indicated an agronomically significant amount of nitrogen being fixed in soil. Kallar grass has a similar growth habitat to rice. Therefore similar studies were carried out with rice after isolating various diazotrophs from the roots which were also screened for their ability to produce auxin (IAA). Five such strains namely Azospirillum lipoferum N-4, Azospirillum brasilense Wb-3, Azoarcus K-1, Pseudomonas 96-51, Zoogloea Ky-1 were selected for inoculating two rice varieties i.e. NIAB-6 and BAS-370 under aseptic laboratory conditions. The nitrogen fixed was quantified using the N isotopic dilution method. Variety BAS-370 had nearly 70% nitrogen derived from atmosphere (Ndfa) when inoculated with Azospirillum N-4. Similar studies with the mixed inoculum using N fertilizer in the micro plots indicated that nearly 29% of plant nitrogen was derived from the atmosphere.

acetylene reduction Azospirillum Azoarcus grasses N isotope dilution root-associated bacterial population 


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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • K.A. Malik
    • 1
  • Rakhshanda Bilal
    • 1
    • 2
  • Samina Mehnaz
    • 1
  • G. Rasul
    • 1
  • M.S. Mirza
    • 1
  • S. Ali
    • 1
  1. 1.National Institute for Biotechnology and Genetic EngineeringFaisalabadPakistan
  2. 2.PINSTECHRawalpindiPakistan

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