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Molecular markers linked to genes underlying seedling tolerance for ferrous iron toxicity


A double haploid (DH) population consisting of 123 lines derived from a japonica variety, Azucena, and an indica variety, IR64, and 100 BC1F1 (Azucena) lines were cultivated hydroponically using two treatments: one with excess Fe2+ at the concentration of 250 mg L-1 and a control with standard nutrient solution. Genotypic tolerance was evaluated using an index scale based on degree of leaf bronzing and relative decease in shoot dry weight (RDSDW) Toxic symptoms were not observed for Azucena and BClFl plants. In contrast, index values for the DH population indicated segregation for tolerance, and IR64 was moderately sensitive. Molecular marker loci associated with variations in index values and in RDSDW, and gene loci for tolerance were detected using 175 Markers mapped on all 12 chromosomes by single marker loci and interval mapping. Two gene loci were identified to be flanked by RG345 and RG381, and linked to RG810, respectively, on chromosome 1 for both index values and RDSDW. They explained 32% and 13% of the total variation in the index values, and 15% and 21 % in the RDSDW in the population, respectively. The variation in RDSDW was also explained by a locus linked to RG978 on chromosome 8 by about 10%. Comparison of the two marker genotypic class means indicated that the tolerant alleles were from Azucena at the first locus on chromosome 1 and the locus on chromosome 8, and that at the second locus on chromosome 1 from IR64.

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  1. Bode K, Doring O, Luthje S, Neue H U and Bottger M 1995 The role of active oxygen in iron tolerance of rice (Oryza sativaL.). Protoplasma 184, 249-255.

    Google Scholar 

  2. Byren P F, McMullen M D, Snook M E, Musket T A, Theuri J M, Widstrom N W, Wiseman B R and Coe E H 1996 Quantitative trait loci and metabolic pathways: Genetic control of the concentration of maysin, a corn earwormresistance factor, in maize silks. Proc. Natl. Acad. Sci. USA. 93, 8820-88225.

    PubMed  Google Scholar 

  3. Causse M, Fulton T M, Cho Y G, Ahn S N, Chunwongse J, Wu K, Xiao J, Yu Z H, Ronald P C, Harrington S B, Second G A, McCouch S R and Tanksley S D 1994 Saturated molecular map of the rice genome, based on an inter-specific backcross population. Genetics 138, 1251-1274.

    PubMed  Google Scholar 

  4. Genon J G, Hepcee N, Duffy J E, Delvaux B and Hennebert P A 1994 Iron and other chemical soil constraints to rice in highland swamps of Burundi. Plant and Soil 166(1), 109-661.

    Google Scholar 

  5. Guiderdoni E, Galinato E, Luistro J and Vergara G 1992 Anther culture of tropical japonica × indica hybrids of rice (Oryza SativaL.). Euphytica 62, 219-224.

    Google Scholar 

  6. Gunawarkena I, Virrnani S S and Sumo F J 1982 Breeding rice for tolerance to iron toxicity. Oryza 19, 5-12.

    Google Scholar 

  7. Huang N, Parco A, Mew T, Magpantay G, McCouch S, Guiderdoni E, Xu J, Subudhi P, Angeles R, Khush GS 1997 RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown plant hopper resistance in a doubled haploid rice population. Molecular Breeding (in press).

  8. International Rice Research Institute (IRRI) 1965 Annual report 1964 Los Banos, Philippines. 335p.

  9. International Rice Research Institute (IRRI) 1996 Standard evaluation system for rice. 4th edition, IRRI, Manila, Philippines. 35p.

    Google Scholar 

  10. Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J, Antonio BA, Shomura A, Shimizu T, Lin S Y, Inoue T, Fukuhara A, Shimano T, Kuboki Y, Toyama T, Miyamoto Y, Kirihara T, Hayasaka K, Miyao A, Monna L, Zhong H S, Tamura Y, Wang Z X, Momma T, Umehara Y, Yano M, Sasaki T and Minobe Y 1994 A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nature Genet. 8, 365-372.

    Article  PubMed  Google Scholar 

  11. Lai K L and Hou C 1976 Studies on the differentiation of physiological characteristics of the roots of different type rice plants (Oryza sativaL.). Taiwan Agricultural Center, Bulletin of the Phytotron, Oct. 10, 17-21.

  12. Lander E S 1993 Mapmaker/Exp 3.0 and Mapmaker/QTL, 1. 1, tutorial and Reference Manual. Whitehead Institute, 9 Cambridge Center, Cambridge MA 02142.

    Google Scholar 

  13. Liao Z W, Lin D J and Wen Z P 1994 Influence of Mn and Fe counteraction on rice (Oryza salivaL.) oranging physiological disease. Pedosphere (China), 4(2), 119-126.

    Google Scholar 

  14. Ota Y 1968 Studies on the occurrence of the physiological disease called “bronzing”. Bull. Natl. Inst. Agric. Sci. (Japan) D 18, 31-104.

    Google Scholar 

  15. Ponnamperuma F N, Bradfield R and Peech M 1955 Physiological disease of rice attributable to iron toxicity, Nature 175, 265.

    Google Scholar 

  16. Riede C R and Anderson J A. 1996 Linkage of RFLP markers to an aluminum tolerance gene in wheat. Crop Sci. 36, 905-909.

    Google Scholar 

  17. Tanksley S D 1993 Mapping polygenes. Annu. Rev. Genet. 27, 205-233.

    PubMed  Google Scholar 

  18. Yoshida S, Forno D A, Cock J H and Gomez K A 1976 Laboratory mannul for physiological studies of rice. The third edition. Int. Rice Res. Inst., Manila, Philippines, pp 61-64.

    Google Scholar 

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Wu, P., Luo, A., Zhu, J. et al. Molecular markers linked to genes underlying seedling tolerance for ferrous iron toxicity. Plant and Soil 196, 317–320 (1997).

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  • ferrous iron toxicity
  • molecular markers
  • Oryza sativa L.