, 108:191 | Cite as

Chromosomal Analysis of Repeated DNAs in the Rainbow Wrasse Coris Julis (Pisces, Labridae)

  • M. Mandrioli
  • M.S. Colomba
  • R. Vitturi


Despite the interest of several authors, the karyotype of the labrid C. julis is still debated and in particular the presence of sex-chromosomes is still contradictory. In order to analyze the karyotype organization of C. julis we have performed an analysis with classical and molecular cytogenetic techniques. Our results after silver-, CMA3- and DAPI-staining, C-banding and fluorescent in situ hybridization (FISH) using 28S rDNA, 5S rDNA, and telomeric repeat (TTAGGG) n as probes allowed us to characterize the chromosomal location of several repetitive DNAs of C. julis. Finally, regardless of the technique used, no difference in the chromosome complement was found between males and females.

28S and 5S rDNA karyotype rainbow wrasse telomere C-banding 


  1. Bacci, G. & A. Razzauti, 1957. Falso gonocorismo in Coris julis L. Rend Accad Lincei Serie VIII 23: 181–189.Google Scholar
  2. Bentivegna, F. & M.B. Rasotto, 1983. Anatomical features of sex-inversion in the rainbow wrasse, Coris julis. Boll. Zool. 50: 73–78.Google Scholar
  3. Cano, J., G. Thode & M.C. Alvarez, 1982. Karyoevolutive considerations in 29 Mediterranean teleost fishes. Vie Milieu 32: 21–24.Google Scholar
  4. Capriglione, T., A. Morescalchi, E. Olmo, L. Rocco & V. Stingo, 1994. Satellite DNAs, heterochromatin and sex chromosomes in Chionodraco hamatus (Channichthydae, Perciformes). Polar Biol. 14: 284–290.CrossRefGoogle Scholar
  5. Caputo, V., M. Sorice, R. Vitturi, R. Magistrelli & E. Olmo, 1998. Cytogenetic studies in some species of Scorpaeniformes (Teleostei; Percomorpha). Chromosome Res. 6: 255–262.PubMedCrossRefGoogle Scholar
  6. Donlon, T.A. & R.E. Magenis, 1983. Methyl green is a substitute for distamycin A in the formation of distamycin A/DAPI C-bands. Hum. Genet. 65: 144–146.PubMedCrossRefGoogle Scholar
  7. Duchac, B., F. Huber, H. Muller & D. Senn, 1982. Mating behaviour and cytogenetical aspects of sex-inversion in the fish Coris julis (Labridae, Teleostei). Experientia 38: 809–811.CrossRefGoogle Scholar
  8. Geiduschek, E.P. & G.P. Tocchini-Valentini, 1988. Transcription by RNA polymerase III. Ann. Rev. Biochem. 57: 873–914.PubMedCrossRefGoogle Scholar
  9. Gornung, E., I. Gabrielli & L. Sola, 1998. Localization of the (TTAGGG)n telomeric sequence in zebrafish chromosomes. Genome 41: 136–138.CrossRefGoogle Scholar
  10. Howell, W.M. & D.A. Black, 1980. Controlled silver-staining of nucleolar organizer region with protective colloidal developer: a 1 step method. Experentia 36: 1014.CrossRefGoogle Scholar
  11. Ijdo, J.W., R.A. Wells, A. Baldini & S.T. Reeders, 1991. Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Res. 19: 4780.PubMedGoogle Scholar
  12. Levan, A., K. Fredga & A.A. Sandberg, 1964. Nomenclature for centromeric position on chromosome. Hereditas 52: 201–220.CrossRefGoogle Scholar
  13. Long, E.O. & I.B. Dawid, 1980. Repeated genes in eukaryotes. Ann. Rev. Biochem. 49: 727–764.PubMedCrossRefGoogle Scholar
  14. Meyne, J., R.J. Baker, H.H. Hobart, T.C. Hsu, O.A. Ryder, O.G. Ward, J.E. Wiley, D.H. Wurster-Hill, T.L. Yates & R.K. Moyzis, 1990. Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes. Chromosoma 99: 3–10.PubMedCrossRefGoogle Scholar
  15. Morán, P., J.L. Martinez, E. Garcia-Vazquez & A.M. Pendás, 1996. Sex chromosome linkage of 5S rDNA in rainbow trout (Oncorhynchus mykiss). Cytogenet. Cell Genet. 75: 145–150.PubMedGoogle Scholar
  16. Pendás, A.M., P. Morán, J.P. Freje & E. Garcia-Vazquez, 1994. Chromosomal mapping and two nucleotide sequences of two tandem repeats of Atlantic salmon 5S rDNA. Cytogenet. Cell Genet. 67: 31–36.PubMedGoogle Scholar
  17. Phillips, R.B. & P.E. lhssen, 1985. Identification of sex chromosomes in lake trout (Salvelinus namaycush). Cytogenet. Cell Genet. 39: 14–18.PubMedGoogle Scholar
  18. Phillips, R.B. & K.M. Reed, 1996. Application of fluorescent in situ hybridization (FISH) techniques to fish genetics: a review. Aquaculture 140: 197–216.CrossRefGoogle Scholar
  19. Pisano, E., S. Rossetti & M. Vacchi, 1994. Karyotypic variability in the Mediterranean rainbow wrasse Coris julis (Linneo, 1758). 8th Congress of Societas Europea Ichthyologorum (SEI), Oviedo (Spain).Google Scholar
  20. Schweizer, D., 1981. Counterstain-enhanced chromosome banding. Hum. Genet. 57: 1–14.PubMedGoogle Scholar
  21. Sola, L., P.J. Monaco & E.M. Rasch, 1990. Cytogenetics of bisexual/unisexual species of Poecilia. I. C-bands, Ag-NOR polymorphisms and sex chromosomes in three populations of Poecilia latipinna. Cytogenet. Cell Genet. 53: 148–154.PubMedCrossRefGoogle Scholar
  22. Sumner, A.T., 1972. A simple technique for demonstrating centromeric heterochromatin. Exp. Cell. Res. 75: 304–306.PubMedCrossRefGoogle Scholar
  23. Tortonese, E., 1975. Fauna d'Italia. Osteichthyes-pesci ossei. (pp. 216–220) Calderini Publisher, Bologna, Italia.Google Scholar
  24. Vitturi, R., P. Carbone, E. Catalano & M. Macaluso, 1984. Chromosome polymorphism in Gobius paganellus, Linneo 1758 (Pisces, Gobiidae). Biol. Bull. 167: 658–668.Google Scholar
  25. Vitturi, R., E. Catalano, A. Maiorca & T. Carollo, 1988. Karyological studies in Coris julis (Pisces, Labridae). Genetica 76: 219–223.PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • M. Mandrioli
    • 1
  • M.S. Colomba
    • 2
  • R. Vitturi
    • 2
  1. 1.Dipartimento di Biologia AnimaleUniversitá di Modena e Reggio EmiliaModenaItaly
  2. 2.Dipartimento di Biologia AnimaleUniversitá di PalermoPalermoItaly

Personalised recommendations