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Detection of ICAM-1 in Experimentally Induced Colitis of ICAM-1-deficient and Wild-type Mice: An Immunohistochemical Study

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Abstract

Adhesion molecules (e.g. ICAM-1, CD 54) are known to be upregulated on activated vascular endothelial cells during inflammatory reactions. To study the role of ICAM-1 in intestinal inflammation in vivo, we induced acute experimental colitis in wild-type (C57BL/6) mice and ICAM-1-deficient mice, by feeding the animals with 3% dextran sodium sulphate (DSS) in drinking water for 7 days. In the control strain the immunohistochemical staining showed a very pronounced endothelial upregulation of ICAM-1 after the DSS treatment observed in areas of inflammatory infiltrate, especially in venules or arterioles of the propria and submucosa, and partly in the mesocolon. DSS-fed ICAM-1-deficient mice showed no endothelial enhancement and only faint staining of venules or capillaries approaching that encountered in the control ICAM-1-deficient animals. Our data indicate that ICAM-1 may play a crucial role in the development of acute intestinal inflammation, consistent with our finding that ICAM-1 deficiency can obviate severe forms of experimentally induced colitis in mice.

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Authors and Affiliations

  1. Division of Immunology and Gnotobiology, Institute of Microbiology, ASCR, Prague, Czech Republic

    Farid Bendjelloul, Pavel Rossmann, Ludmila Tučková & Helena Tlaskalová-Hogenová

  2. Department of Mammalian Development, Institute of Molecular Genetics, ASCR, Prague, Czech Republic

    Petr Malý

  3. 1st Faculty of Medicine, Charles University, ASCR, Prague, Czech Republic

    Václav Mandys, Marie Jirkovská & Ludmila Prokešová

  4. Institute of Experimental Medicine, ASCR, Prague, Czech Republic

    Václav Mandys

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  1. Farid Bendjelloul
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  2. Pavel Rossmann
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Bendjelloul, F., Rossmann, P., Malý, P. et al. Detection of ICAM-1 in Experimentally Induced Colitis of ICAM-1-deficient and Wild-type Mice: An Immunohistochemical Study. Histochem J 32, 703–709 (2000). https://doi.org/10.1023/A:1004191825644

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