Abstract
The human oviduct epithelium primarily consists of ciliated cells and secretory cells. Solitary cilia usually extend from the apical surface of the secretory cells. We investigated the localization of γ-tubulin in the ciliary basal apparatus of both cell types by fluorescence immunohistochemistry and immunoelectron microscopy. In addition to basal bodies, γ-tubulin was identified in the lateral basal foot, especially the basal foot cap. This observation is consistent with previous observations that microtubules radiate from the basal foot and the basal foot serves as the microtubule organizing centre.
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References
Anderson RGW (1972) The three-dimensional structure of the basal body from the rhesus monkey oviduct. J Cell Biol 54: 246-265.
Boisvieux-Ulrich E, Sandoz D (1991) Determination of ciliary polarity precedes differentiation in the epithelial cells of quail oviduct. Biol Cell 72: 3-14.
Burton PR (1992) Ultrastructural studies of microtubules and microtubule organizing centers of vertebrate olfactory neuron. Microsc Res Tech 15: 142-156.
Hagiwara H, Aoki T, Ohwada N, Fujimoto T (2000) Identification of a 195 kDa protein in the striated rootlet: its expression in ciliated and ciliogenic cells. Cell Motil Cytoskeleton 45: 200-210.
Harrisson F (1989) Primary cilia associated with striated rootlets in granulated and folliculo-stellate cells of the avian adenohypophysis. Anat Embryol 180: 543-547.
Liang A, Ruiz F, Heckmann K, Klotz C, Tollon Y, Beisson J, Wright M (1996) Gamma-tubulin is permanently associated with basal bodies in ciliates. Eur J Cell Biol 70: 331-338.
Muresan V, Joshi HC, Besharse JC (1993) γ-Tubulin in differentiated cell type: localization in the vicinity of basal bodies in retinal photoreceptors and ciliated epithelia. J Cell Sci 104: 1229-1237.
Odor DL, Blandau RJ (1985) Observations on the solitary cilium of rabbit oviductal epithelium: its motility and ultrastructure. Am J Anat 174: 437-453.
Palacios MJ, Joshi HC, Simerly C, Schatten G (1993) Gamma-tubulin reorganization during mouse fertilization and early development. J Cell Sci 104: 383-389.
Pitelka DR (1974) Basal bodies and root structures. In: Sleigh MA, ed. Cilia and flagella, New York: Academic Press, pp. 437-469.
Reed W, Avolio J, Satir P (1984) The cytoskeleton of the apical border of the lateral cells of freshwater mussel gill: structural integration of microtubule and actin filament-based organelles. J Cell Sci 68: 1-33.
Schiebel E (2000) γ-Tubulin complexes: binding to centrosome, regulation and microtubule nucleation. Curr Opin Cell Biol 12: 113-118.
Scott V, Sherwin T, Gull K(1997) γ-Tubulin in trypanosomes: molecular characterization and localisation to multiple and diverse microtubule organising centres. J Cell Sci 110: 157-168.
Silflow CD, Liu B, LaVoie M, Richardson EA, Palevitz BA (1999) γ-Tubulin in Chlamydomonas: characterization of the gene and localization of the gene product in cells. Cell Motil Cytoskeleton 42: 285-297.
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Hagiwara, H., Kano, A., Aoki, T. et al. Localization of γ–tubulin to the Basal Foot Associated with the Basal Body Extending a Cilium. Histochem J 32, 669–671 (2000). https://doi.org/10.1023/A:1004163315822
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DOI: https://doi.org/10.1023/A:1004163315822