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Genetica

, 108:253 | Cite as

Chromosomal Location of Ribosomal DNA (rDNA), (GATA)n and (TTAGGG)n Telomeric Repeats in the Neogastropod Fasciolaria Lignaria (Mollusca: Prosobranchia)

  • R. Vitturi
  • M.S. Colomba
  • P. Gianguzza
  • A.M. Pirrone
Article

Abstract

This paper reports on a successful application of fluorescent in situhybridization (FISH) with three repetitive DNA probes (ribosomal DNA (rDNA), (GATA)nand (TTAGGG)n) in the chromosomes of Fasciolaria lignaria(Mollusca: Prosobranchia: Neogastropoda). rDNA FISH consistently identified four chromosome pairs per spread in the three examined specimens. The telomeric sequence (TTAGGG)nhybridized with termini of all chromosomes. GATA FISH revealed abundant, dispersed minisatellite regions which were not associated to the XY sex-determining mechanism as indicated by the absence of a Y specific pattern of labelling.

Mollusca repetitive DNA Neogastropoda Fasciolaria lignaria FISH 

References

  1. Cantone, M., R. Barbieri, G. Duro, V. Izzo & G. Giudice, 1993. Sequence analysis of the rDNA spacer of Paracentrotus lividusand observations about pre-DNA processing. Mol. Biol. 18: 177–182.Google Scholar
  2. Colomba, M.S., R. Vitturi & M. Zunino, 2000. Karyotype analysis, banding, and fluorescent in situhybridization in the scarab beetle Gymnopleurus sturmiMcLeay (Coleoptera Scarabaeoeidea: Scarabaeidae). J. Hered. 91 (3): 260–264.PubMedCrossRefGoogle Scholar
  3. Demas, S. & S. Wachtel, 1990. DNA fingerprinting in reptiles: Bkm hybridization patterns in Crocodilia and Chelonia. Genome 34: 472–476.Google Scholar
  4. Durbin, E.J., J.R. Stalvey & R.P. Erickson, 1989. Northern analyses using single-stranded probes do not support a role for GATA/GACA repeats in sex determination in mice and men. Mol. Reprod. Dev. 1(2): 116–121.PubMedCrossRefGoogle Scholar
  5. Epplen, J.T., 1988. On the simple repeated GATA sequences in animal genomes: a critical reappraisal. J. Hered. 79: 409–417.PubMedGoogle Scholar
  6. Gonzáles-Tizón, A., A. Martínez-Lage, L. Mariñas, R. Feire, L. Cornudella & Y. Méndez, 1998. Cytogenetic characterization of Donax trunculus(Mollusca, Bivalvia). 13th International Chromosome Conference. Cytogenet. Cell Genet. Abstracts 109.Google Scholar
  7. Gortner, G., M. Nenno, K. Weising, D. Zink, W. Nagl & G. Kahl, 1998. Chromosomal localization and distribution of simple sequence repeats and the Arabidopsis-type telomere sequence in the genome of Cicer arietinumL. Chrom. Res. 6: 97–104.PubMedCrossRefGoogle Scholar
  8. Guo, X. & S.K. Allen, Jr., 1997. Fluorescence in situhybridization of vertebrate telomere sequence to chromosomes ends of the pacific oyster, Crassostrea gigasThunberg. J. Shellfish Res. 16 (1): 87–89.Google Scholar
  9. Hinegardner, R., 1974. Cellular DNA content of the Mollusca. Comp. Biochem. Physiol. 47 A: 447–460.CrossRefGoogle Scholar
  10. Ijdo, J.W., R.A. Wells, A. Baldini & S.T. Reeders, 1991. Improved telomere detection using a telomere repeat probe (TTAGGG)ngenerated by PCR. Nucl. Ac. Res. 19: 4780.Google Scholar
  11. Jha, A.N., I. Dominquez, A.S. Balajee, T.H. Hutchinson, D.R. Dixon & A.T. Natarajan, 1995. Localization of vertebrate telomeric sequence in the chromosomes of two marine worms (phylum Annelida: class Polychaeta). Chrom. Res. 3: 507–508.PubMedCrossRefGoogle Scholar
  12. Jones, K.W. & L. Singh, 1985. Snakes and the evolution of sex chromosomes. Trends Genet. 1: 55–61.CrossRefGoogle Scholar
  13. Levan, A., K. Fredga & A.A. Sandberg, 1964. Nomenclature for centromeric position of chromosomes. Hereditas 52: 201–220.CrossRefGoogle Scholar
  14. Mason, J.M. & H. Biessman, 1995. The unusual telomeres of Drosophila. Trends Genet. 11: 58–62.PubMedCrossRefGoogle Scholar
  15. Meyne, J., R.J. Baker, H.H. Hobart, T.C. Hsu, A.R. Oliver, O.G. Ward et al., 1990. Distribution of non-telomeric sites of the (TTAGGG)ntelomeric sequences in vertebrate chromosomes. Chromosoma 99: 3–10.PubMedCrossRefGoogle Scholar
  16. Miklos, G.L.G., K.I. Matthaei & K.C. Reed, 1989. Occurrence of the (GATA)nsequences in vertebrate and invertebrate genomes. Chromosoma 98: 194–200.PubMedCrossRefGoogle Scholar
  17. Nanda, I., M. Schartl, W. Feichtinger, J.T. Epplen & M. Schmid, 1992. Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences. Chromosoma 101(5,6): 301–310.PubMedCrossRefGoogle Scholar
  18. Okazaki, S., K. Tsuchida, M. Maekawa, M. Ishikawa & H. Fujiwara, 1993. Identification of a pentanucleotide telomeric sequence (TTAGG)nin the silkworm Bombix moriand in other insects. Mol. Cell Biol. 13: 1424–1432.PubMedGoogle Scholar
  19. Pascoe, P.L., S.J. Patton, R. Critcher & D.R. Dixon, 1996. Robertsonian polymorphism in the marine gastropod Nucella lapillus: advances in karyology using rDNA loci and NORs. Chromosoma 104(6): 455–460.PubMedGoogle Scholar
  20. Pelliccia, F., M. Di Castro, V. Lanza, E.V. Volpi & A. Rocchi, 1991. GATA repeats in the genome of Asellus aquaticus(Crust. Isop.) Chromosoma 100: 152–155.PubMedCrossRefGoogle Scholar
  21. Pelliccia, F., E.V. Volpi, V. Lanza, L. Gaddini, A. Baldini & A. Rocchi, 1994. Telomeric sequences of Asellus aquaticus(Crust. Isop.). Heredity 72: 78–80.Google Scholar
  22. Pendás, A.M., P. Morón & E. García-Vásquez, 1993. Multichromosomal location of ribosomal RNA genes and heterochromatin association in brown trout. Chrom. Res. 1: 63–67.PubMedCrossRefGoogle Scholar
  23. Phillips, R.B. & K.M. Reed, 1996. Application of fluorescence in situhybridization (FISH) tecniques to fish genetics: a review. Aquaculture 140: 197–216.CrossRefGoogle Scholar
  24. Sabelli, B. & G. Spada, 1981. Guida illustrata all'iden-tificazione delle conchiglie del Mediterraneo. Boll Malac (suppl) XVII (3, 4) GI 18.Google Scholar
  25. Sahara, K., F. Marec & W. Traut, 1999. TTAGG telomere repeats in chromosomes of some insects and other arthropods. Chrom. Res. 7: 449–460.PubMedCrossRefGoogle Scholar
  26. Sánchez, A., R. Jímenez, M. Burgos, S. Stitou, F. Zurita & R. Díaz De La Guardia, 1995. Cytogenetic peculiarities in the Algerian hedgehog: silver stains not only NORs but also heterochromatic blocks. Heredity 75: 10–16.PubMedGoogle Scholar
  27. Singh, L., C. Phillips & K.W. Jones, 1984. The conserved nucleotide sequences of Bkm, which define Sxr in the mouse, are transcribed. Cell 36: 111–120.PubMedCrossRefGoogle Scholar
  28. Thiriot-Quievreux, C. & A. Insua, 1982. Les karyotypes de quelques espèce de bivalves et de gastèropode marins. Mar. Biol. 70: 165–172.CrossRefGoogle Scholar
  29. Tiersch, T.R., B.A. Simco, K.B. Davis & S.S. Wachtel, 1992. Molecular genetics of sex determination in channel catfish: studies on SRY, ZFY, Bkm, and human telomeric repeats. Biol. Reprod. 47(2): 185–192.PubMedCrossRefGoogle Scholar
  30. Trask, B., 1991. Fluorescence in situhybridization: application in cytogenetics and gene mapping. Trends Genet. 7(5): 149–154.PubMedCrossRefGoogle Scholar
  31. Traut, W., 1987. Hypervariable Bkm DNA loci in a moth Ephestia kuehniella: does transposition cause restriction fragment length polymorphism? Genetics 115: 112–118.Google Scholar
  32. Vitturi, R. & E. Catalano, 1990. Spermatocyte chromosome banding studies in Buccinulum corneum(Prosobranchia: Neogastropoda): variation in silver-NOR banding pattern. Mar. Biol. 104: 259–263.CrossRefGoogle Scholar
  33. Vitturi, R., E. Catalano, M. Macaluso & A. Maiorca, 1987. Spermatocyte chromosomes in six species of Neogastropoda (Mollusca, Prosobranchia). Biol. Zentrbl. 106: 81–88.Google Scholar
  34. Vitturi, R., M.S. Colomba, R. Barbieri & M. Zunino, 1999. Ribosomal DNA location in the scarab beetle Thorectes intermedius(Costa) (Coleoptera: Geotrupidae) using banding and fluorescent in situhybridization. Chrom. Res. 7: 1–6.CrossRefGoogle Scholar
  35. Vitturi, R., M.S. Colomba, V. Caputo & A. Pandolfo, 1998. XY chromosome sex system in the neogastropods Fasciolaria lignariaand Pisania striata(Mollusca: Prosobranchia). J. Hered. 89(6): 538–543.CrossRefGoogle Scholar
  36. Vitturi, R., P. Gianguzza, M.S. Colomba, K.R. Jensen & S. Riggio, in press. Cytogenetics in the sacoglossan Oxynoe olivaceaRafinesque, 1819 (Mollusca: Opisthobranchia): karyotype, chromosome banding and fluorescent in situhybridization. Mar. Biol.Google Scholar
  37. Vitturi, R., M.S. Colomba, A. Pirrone & A. Libertini, 2000. Physical mapping of rDNA genes, (TTAGGG)ntelomeric sequence and other karyological features in two earthworms of the family Lumbricidae (Annelida: Oligochaeta). Heredity 85: 203–207.PubMedCrossRefGoogle Scholar
  38. Wachtel, S., S. Demas, T. Tiersch, P. Pechan & D. Shapiro, 1991. Bkm satellite DNA and ZFY in the coral reef fish Anthias squamipinnis. Genome 34 (4): 612–617.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • R. Vitturi
    • 1
  • M.S. Colomba
    • 1
  • P. Gianguzza
    • 1
  • A.M. Pirrone
    • 2
  1. 1.Dipartimento di Biologia AnimaleUniversità di PalermoPalermoItaly
  2. 2.Dipartimento di Biologia Cellulare e dello Sviluppo ‘A. Monroy’Università di PalermoPalermoItaly

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