Abstract
The ultracytochemical localization of membrane-bound guanylate cyclases A and C, stimulated by atrial natriuretic peptide and guanylin respectively, has been studied in human sweat glands. The results showed that the peptides stimulated guanylate cyclases A and C in both eccrine and apocrine glands. In the secretory cells, enzymatic activity was present on the plasma membranes and on intracellular membranes involved in the secretory mechanism. In eccrine glands, the cells of the excretory duct also presented enzymatic activity on the plasma membranes. In both glands, myoepithelial cells, surrounding the secretory cells, exhibited only guanylate cyclase A activity. These localizations of enzymatic activity suggest a role for both atrial natriuretic peptide and guanylin in regulating glandular secretion.
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Bianciotti LG, Elverdin JC, Vatta MS, Colatrella C, Fernández BE (1994) Atrial natriuretic factor enhances induced salivary secretion in the rat. Regul Pept 49: 195-202.
Bianciotti LG, Elverdin JC, Vatta MS, Fernández BE (1996) Atrial natriuretic factor modifies the composition of induced-salivary secretion in the rat. Regul Pept 65: 139-143.
Crawford I, Malone PC, Zeitlin PL, Guggino WB, Hyde SC, Turley H, Gatter KC, Harris A, Higgins CF (1991) Immunocytochemical localization of the cystic fibrosis gene product CFTR. Cell Biol 88: 9262-9266.
Currie MG, Fok KF, Kato J, Moore RJ, Hamra FK, Duffin KL, Smith CE (1992) Guanylin: An endogenous activator of intestinal guanylate cyclase. Proc Natl Acad Sci USA 89: 947-951.
Drewett JG, Garbers DL (1994) The family of guanylyl cyclase receptors and their ligands. Endocrine Rev 15: 135-162.
Fan X, Hamra FK, London RM, Eber SL, Krause WJ, Freeman RH, Smith CE, Currie MG, Forte LR (1997a) Structure and activity of uroguanylin and guanylin from the intestine and urine of rats. Am J Physiol 273: E957-E964.
Fan X, Wang J, London RM, Eber SL, Krause WJ, Freeman RH, Forte LR (1997b) Signaling pathways for guanylin and uroguanylin in the digestive, renal, central nervous, reproductive and lymphoid systems. Endocrinology 138: 4636-4648.
Fernández BE, Bianciotti LG, Vatta MS, Dominguez AE (1993) Atrial natriuretic factor modifies bile flow and composition in the rat. Regul Pept 43: 177-184.
Fonteles MC, Greenberg RN, Monteiro HS, Currie MG, Forte LR (1998) Natriuretic and kaliuretic activities of guanylin and uroguanylin in the isolated perfused rat kidney. Am J Physiol 275: F191-F197.
Forte LR, Currie MG (1995) Guanylin: a peptide regulator of epithelial transport. FASEB J 9: 643-650.
Goraczniak RM, Duda T, Sharma RK (1992) A structural motif that defines the ATP-regulatory module of guanylate cyclase in atrial natriuretic factor signalling. Biochem J 282: 533-537.
Greger R (1996) The formation of sweat. In: Greger R, Windhorst U, eds. ComprehnsiveHumanPhysiology Vol. 2. Berlin, Heidelberg: Springer-Verlag pp. 2219-2228.
Guba M, Kuhn M, Forssmann W-G, Classen M, Gregor M, Seidler U (1996) Guanylin strongly stimulates rat duodenal HCO -3 secretion: proposed mechanism and comparison with other secretagogues. Gastroenterology 111: 1558-1568.
Hamra FK, Eber SL, Chin DT, Currie MG, Forte LR (1997) Regulation of intestinal uroguanylin/guanylin receptor-mediated responses by mucosal acidity. Proc Natl Acad Sci USA 94: 2705-2710.
Hamra FK, Krause WJ, Eber SL, Freeman RH, Smith CE, Currie MG, Forte LR (1996) Opossum colonic mucosa contains uroguanylin and guanylin peptides. Am J Physiol 270: G708.
Kang JH, Sahai A, Criss WE, West WL (1982) Ultracytochemical localization of estrogen-stimulated guanylate cyclase in rat uterus. J Histochem Cytochem 30: 331-342.
Khare S, Wilson D, Wali RK, Tien X-Y, Bissonnette M, Niedziele SM, Bolt JG, Sitrin MD, Brasitus TA (1994) Guanylin activates rat colonic particulate guanylate cyclase. Biochem Biophys Res Commun 203: 1432-1437.
Marala RB, Duda T, Goraczniak RM, Sharma RK (1992) Genetically tailored atrial natriuretic factor-dependent guanylate cyclase. Immunological and functional identity with 180 kDa membrane guanylate cyclase and ATP signaling site. FEBS Lett 296: 254-258.
Marala RB, Sitaramayya A, Sharma RK (1991) Dual regulation of natriuretic factor-dependent guanylate cyclase activity by ATP. FEBS Lett 281: 73-76.
Matsushita K, Nishida Y, Hosomi H, Tanaka S (1991) Effects of atrial natriuretic peptide on water and NaCl absorption across the intestine. Am J Physiol 260: R6-R12.
Mörk AC, Hongpaisan J, Roomans GM (1995) Ion transport in primary cultures from human sweat gland coils studied with X-ray microanalysis. Cell Biol Int 19: 151-159.
O'Donnell ME (1989) Regulation of Na-K-Cl cotransport in endothelial cells by atrial natriuretic factor. Am J Physiol 257: C36-C44.
Poeggel G, Luppa H (1988) Histochemistry of nucleotidyl cyclase and cyclic nucleotide phosphodiesterases. Histochem J 20: 249-268.
Rambotti MG, Ercolani P, Spreca A (1993) Ultracytochemical localization of particulate guanylate cyclase in rat adrenal gland exposed to stimulation by porcine brain natriuretic peptide. Histochem J 25: 384-391.
Rambotti MG, Giambanco I, Spreca A (1997) Detection of guanylate cyclases A and B stimulated by natriuretic peptides in gastroenteric tract of rat. Histochem J 29: 117-126.
Rambotti MG, Giambanco I, Spreca A (2000) Ultracytochemical detection of guanylate cyclase-C activity in alimentary tract and associated glands of rat. Influence of pH, ATP and ions Mg2+ and Mn2+. Histochem J (in press).
Rambotti MG, Spreca A (1991) Ultracytochemical demonstration of guanylate cyclase in rat lung after incubation by ANF. Cell Mol Biol 37: 455-462.
Reddy MM, Quinton PM (1994) Rapid regulation of electrolyte absorption in sweat duct. J Membr Biol 140: 57-67.
Scavone C, Scanlon C, McKee M, Nathanson JA (1995) Atrial natriuretic peptide modulates sodium and potassium-activated adenosine triphosphate through a mechanism involving cyclic GMP and cyclic GMP-dependent protein kinase. J Pharmacol Exp Ther 272: 1036-1043.
Schulze W, Krause EG (1983) Cytochemical demonstration of guanylate cyclase activity in cardiac muscle. Histochemistry 77: 243-254.
Scott RB, Maric M (1991) The effect of atrial natriuretic peptide on small intestine contractility and transit. Peptides 12: 799-803.
Skofitsch G, Jacobowitz DM (1988) Atrial natriuretic peptide in the central nervous system. Cell Mol Neurobiol 8: 339-391.
Spreca A, Giambanco I, Rambotti MG (1998) Guanylate cyclase-A in sebaceous glands. It J Anat Embriol (suppl) 103: 168.
Spreca A, Giambanco I, Rambotti MG (1999) Ultracytochemical study of guanylate cyclase A and B in light-and dark-adapted retinas. Histochem J 31: 477-483.
Spreca A, Rambotti MG (1994) Ultracytochemical localization of particulate guanylate cyclase after stimulation with natriuretic peptides in lamb olfactory mucosa. Histochem J 26: 778-786.
Waldman SO, Rapoport RM, Murad F (1984) Atrial natriuretic factor selectively activates particulate guanylate cyclase and elevates cyclic GMP in rat tissues. J Biol Chem 259: 14332-14334.
Yeung VTF, Lai CK, Cockram CS, Joung JD, Nicholls MG (1991) Binding of brain and atrial natriuretic peptides to cultured mouse astrocytes and effect on cyclic GMP. J Neurochem 56: 1684-1689.
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Spreca, A., Simonetti, S. & Grazia Rambotti, M. Atrial Natriuretic Peptide and Guanylin-activated Guanylate Cyclase Isoforms in Human Sweat Glands. Histochem J 32, 725–731 (2000). https://doi.org/10.1023/A:1004149010623
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DOI: https://doi.org/10.1023/A:1004149010623